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SCT044 BioTracker LYSO-TP Live Cell Dye

Live cell imaging dye for hypochlorous acid (HOCl) targeted to cellular lysosomes used to detect phagocytosis, inflammation and cellular apoptosis.

BioTracker LYSO-TP Live Cell Dye MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Based Assays
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SCT044
1 mg  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      Cell Based Assays
      Description
      Catalogue NumberSCT044
      DescriptionBioTracker LYSO-TP Live Cell Dye
      OverviewHypochlorous acid (HOCl) is a highly potent reactive oxygen species (ROS) and helps eliminate pathogens in the innate immune system. Mounting evidence indicates that intracellular HOCl plays additional important roles in regulating inflammation and cellular apoptosis. Subcellular detection of HOCL is currently limited due to low concentration, strong oxidization, and short lifespan of the analyte.

      The BioTracker LYSO-TP dye is a live cell two-photon green fluorescent “turn-on” imaging probe for HOCL. The probe targets HOCL specifically in the lysosomes of cells. The probe exhibit fast response times, good selectivity, and high sensitivity towards hypochlorous acid in living cells. Acedan was chosen as the fluorescence reporting group due to its excellent photophysical properties resulting from the typical “push−pull” (amineketone) structure. Reaction of the probe with HOCl, which deprotects the oxathiolane/mercaptal group to reveal the ketone, would lead to fluorescence enhancement. To monitor subcellular HOCL levels, morpholine was introduced at a long distance from the reaction site to ensure the probe’s intracellular localization.

      Spectral Properties
      Absorbance: 375nm
      Emission: 500nm

      References
      Chang YT et al. Development of Targetable Two-Photon Fluorescent Probes to Image Hypochlorous Acid in Mitochondria and Lysosome in Live Cell and Inflamed Mouse. Model. J Am Chem Soc. 2015 May 13;137(18):5930-8.
      Alternate Names
      • Live cell imaging probe
      References
      Product Information
      Detection methodFluorescence
      PresentationLyophilized
      Applications
      ApplicationLive cell imaging dye for hypochlorous acid (HOCl) targeted to cellular lysosomes used to detect phagocytosis, inflammation and cellular apoptosis.
      Key Applications
      • Cell Based Assays
      Application NotesLive cell fluorescent imaging
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssurancePurity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
      Molar Mass: 357.49 g/mol
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker LYSO-TP Live Cell Dye at -20°C, desiccate and protect from light
      Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT044 04054839532382