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SCT079 BioTracker CoxFluor Live Cell Dye

Fluorescent probe for monitoring COX-2 activity in live cells by confocal microscopy or flow cytometry.

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SCT079
1 mg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT079
      DescriptionBioTracker CoxFluor Live Cell Dye
      OverviewCyclooxygenase (COX), also known as Prostaglandin-endoperoxide synthase (PTGS), is the key enzyme in prostaglandin biosynthesis. There are two isozymes of cyclooxygenase: a constitutive COX-1 and an inducible COX-2, which differ in their regulation of expression and tissue distribution. COX-2 overexpression is prominent in inflammatory diseases, neurodegenerative disorders, and cancer. Directly monitoring COX-2 activity within its native environment poses an exciting approach to investigate the effect of the local environments on protein activity (1).

      BioTracker CoxFluor Live Cell Dye is an isoform selective activity-based sensor for COX-2. The probe may be used for COX-2 inhibitor screening assays and for monitoring COX-2 activity in live cells by confocal microscopy or flow cytometry. CoxFluor strategically links a natural substrate with a dye precursor to engage both the cyclooxygenase and peroxidase activities of COX-2. This catalyzes the release of resorufin and the natural product, as supported by molecular dynamics and ensemble docking. CoxFluor enabled the detection of oxygen dependent changes in COX-2 activity that are independent of protein expression within live macrophage cells (1).

      Spectral Properties
      Absorbance: 540 nm
      Emission: 590 nm

      Reference:
      1. Yadav, A. K., Reinhardt, C. J., Arango, A. S., Huff, H. C., Dong, L., Malkowski, M. G., ... & Chan, J. (2020). An Activity‐Based Sensing Approach for the Detection of Cyclooxygenase‐2 in Live Cells. Angewandte Chemie, 132(8), 3333-3340.
      Alternate Names
      • Live Cell Imaging Probe
      References
      Product Information
      Detection methodFluorescence
      PresentationLyophilized. Off-white solid.
      Applications
      ApplicationFluorescent probe for monitoring COX-2 activity in live cells by confocal microscopy or flow cytometry.
      Key Applications
      • Cell Based Assays
      Application NotesLive cell fluorescent imaging
      Biological Information
      Molecular Weight501.66
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssurancePurity ≥ 95% confirmed by HPLC. Identification confirmed by HNMR, LC-MS, and elemental analysis.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C, desiccate and protect from light
      Note: Centrifuge vial briefly to collect contents at bottom of vial before opening
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT079 04061841827993

      Documentation