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14-557M B-Raf (V599E) Protein, active, 250 µg

Active, N-terminal GST-tagged recombinant human B-Raf residues 416-end, containing a V599E mutation, for use in Kinase Assays.

B-Raf (V599E) Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-557M
250 µg  Also available in 10 μg size (cat#14-557) and in bulk (cat# 14-557-K).
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      Overview

      Description
      Catalogue Number14-557M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionB-Raf (V599E) Protein, active, 250 µg
      OverviewN-terminal GST-tagged recombinant human B-Raf residues 416-end, containing a V599E mutation (also designated V600E)
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal GST-tagged recombinant human B-Raf residues 416-end, containing a V599E mutation, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Gene Symbol
      • BRAF
      • BRAF1
      • B-Raf
      • MGC126806
      • RAFB1
      • p94
      • MGC138284
      • B-raf-1
      • BRAF V600E
      • B-RAF V600E
      Protein TargetB-Raf
      Purification MethodGlutathione agarose affinity chromatography
      Target Sub-FamilyTKL
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15056 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
      COFACTOR: Binds 2 zinc ions per subunit (By similarity).
      SIZE: 766 amino acids; 84437 Da
      SUBUNIT: Interacts with RIT1 (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Brain and testis.
      DISEASE: SwissProt: P15056 # Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. & Defects in BRAF are involved in a wide range of cancers. & Defects in BRAF are involved in lung cancer [MIM:211980]. & Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. & Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
      SIMILARITY: SwissProt: P15056 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
      Molecular Weight67.3kDa
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of MBP substrate in vitro
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-557) and in bulk (cat# 14-557-K).
      Global Trade Item Number
      Catalogue Number GTIN
      14-557M 04053252366512