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ABE1472 Anti-phospho eIF4B Antibody (Ser422)

Anti-phospho eIF4B Antibody (Ser422) | ABE1472 is an antibody against phospho eIF4B Antibody for use in Western Blotting.

Anti-phospho eIF4B Antibody (Ser422) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: EIF4B UniProt Number: P23588
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ABE1472
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1472
      DescriptionAnti-phospho eIF4B Antibody (Ser422)
      Alternate Names
      • Eukaryotic translation initiation factor 4B
      • eIF-4B
      Background InformationEukaryotic translation initiation factor 4B (eIF-4B), encoded by the gene name ELF4B, is an RNA-binding protein that is involved in translation of RNA molecules and consequent protein synthesis. ELF4B is required for the binding of mRNA to ribosomes. ELF4B also functions in close association with EIF4-F and EIF4-A. ELF4B has been demonstrated to bind near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. ELF4B promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. ELF4B contains multiple domains, including an N-terminal RNA recognition motif (RRM) and a C-terminal Arginine Rich Motif (ARM) that facilitate RNA binding. eIF4B may play a specific role in the assembly of the 48S initiation complex. Its translational activity is enhanced by phosphorylation of its ser406 and ser422 residues via the Ras-MAPK and PI3-K/mTOR pathways. eIF4B specially targets RNA molecules involved in cell proliferation and survival in response to signals converging from these pathways.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho eIF4B Antibody (Ser422) | ABE1472 is an antibody against phospho eIF4B Antibody for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human phospho eIF4B (Ser422) near the C-terminus.
      EpitopeNear C-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Bovine, Zebrafish based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EIF4B
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~75 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected phospho eIF4B (Ser422) in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1472 04055977123753