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MABE939 Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody

This Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody is validated for use in Western Blotting and Immunocytochemistry and ELISA for the detection of phospho Histone H3 (Ser10).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: H3F3A, H3.3A, H3F3 UniProt Number: P84243
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MABE939
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, ELISARPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE939
      Replaces04-1093
      DescriptionAnti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody
      Alternate Names
      • H3S10P
      • Histone H3 (phospho Ser10)
      • H3 histone, family 3A
      • H3.3A
      • H3 histone, family 3B
      • H3.3B
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3.3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody is validated for use in Western Blotting and Immunocytochemistry and ELISA for the detection of phospho Histone H3 (Ser10).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • ELISA
      Application NotesImmunocytochemistry Analysis: A 1:5000 dilution from a representative lot detected Histone H3 (Ser10) in HeLa cells (Prof. Taro Tachibana, Osaka City University).
      ELISA Analysis: A representative lot detected Histone H3 (Ser10) using differently phosphorylated and unphosphorylated peptides unmodified H3, H3 S10ph, and H3 S28ph (Prof. Taro Tachibana, Osaka City University).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Histone H3 (Ser10).
      Clone6G8B7
      Concentration1 mg/mL
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • H3F3A
      • H3.3A
      • H3F3
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Histone H3 (Ser10) in 10 µg of nocodazole treated HeLa cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE939 04053252977657

      Documentation

      Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho Histone H3 (Ser10), -2687870 2687870
      Anti-phospho Histone H3 (Ser10), clone 6G8B7 - Q2433686 Q2433686
      Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 3234485 3234485
      Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 3398037 3398037
      Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 3485890 3485890
      Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 3532435 3532435
      Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 3792974 3792974
      Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 3925334 3925334
      Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 3925343 3925343
      Anti-phospho Histone H3 (Ser10), clone 6G8B7 - 4026459 4026459
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