Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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This Anti-phospho Histone H2A.X (Ser139) antibody, clone JBW301, Alexa Fluor® 488 Conjugate is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).
More>>This Anti-phospho Histone H2A.X (Ser139) antibody, clone JBW301, Alexa Fluor® 488 Conjugate is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139). Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Histone H2A.X is a variant of histone H2A, and is similarly associated with genomic DNA. However, histone is structurally different from other members of the H2A family in possessing a C-terminal tail that contains the Ser139 residue that is phosphorylated in response to breaks in double-stranded DNA. The phosphorylation of H2A.X is a rapid process that is mediated by ATM/ATR proteins.
Product Information
Format
AlexaFluor®488
Presentation
Purified mouse conjugate in buffer containing PBS, 15 mg/mL BSA, and 0.1% Sodium Azide.
This Anti-phospho Histone H2A.X (Ser139) antibody, clone JBW301, Alexa Fluor® 488 Conjugate is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).
Key Applications
Immunocytochemistry
Biological Information
Immunogen
Linear peptide corresponding to phospho-Histone H2A.X (Ser139).
Clone
JBW301
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Vertebrates based on 100% sequence homology.
Evaluated by Immunocytochemistry in untreated (negative control) and Staurosporin treated HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Histone H2A.X (Ser139) in Staurosporin treated HeLa cells, and not in untreated HeLa cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
EMD Millipore's Alexa Fluor® Conjugated Histone and puromycin antibodies are validated in immunocytochemistry. All EMD Millipore antibodies are based on the expertise of Upstate & Chemicon. Learn More >>
