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OP33 Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620)

This Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-5) (Wild type).

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OP33
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, Pm, RMMonoclonal Antibody

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
OP33-100UG
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      OP33-20UG
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      Fulfillment and delivery delayed Fulfillment and delivery delayed
      In Stock 
      Discontinued
      Limited Quantities Available
      Availability to be confirmed
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          		 Plastic ampoule 20 μg
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          Description
          OverviewRecognizes the ~53 kDa wild-type p53 protein in its native conformation in Hs27 cells and breast carcinoma tissue. Does not recognize mutant or denatured p53 protein.
          Catalogue NumberOP33
          Brand Family Calbiochem®
          References
          ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
          Ball, R.K., et al. 1984. EMBO J. 3, 1485.
          Product Information
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Positive controlHs27 cells or breast carcinoma tissue
          Preservative≤0.1% sodium azide
          Quality LevelMQ100
          Applications
          Application ReferencesImmunoprecipitation Lu, X., et al. 1992. Cell 70, 153. Frozen Sections Kramata, P., et al. 2005. Cancer Res. 65, 3577. Cordon-Cardo, C., et al. 1991. Cancer Res. 54, 794.
          Key Applications Frozen Sections
          Immunofluorescence
          Immunoprecipitation
          Not Western Blot
          Paraffin Sections
          Application NotesFrozen Sections (see applications references)
          Immunoblotting (not recommended)
          Immunofluorescence (1-20 μg/ml)
          Immunoprecipitation (1 μg per sample)
          Paraffin Sections (5 μg/ml, heat pre-treatment required)
          Application CommentsWild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met(≤ 1 hr) will help reduce background. p53 (Ab-5) may also be used to visualize p53 in cytospins or cultured cells using standard horseradish peroxidase or immunofluorescence detection techniques. p53 (Ab-5) preferentially immunoprecipitates wild-type p53; it should not precipitate mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          ImmunogenvLM mouse tumor cells
          ImmunogenMouse
          ClonePAb1620
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Bovine
          • Human
          • Mouse
          • Primate
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Global Trade Item Number
          Catalogue Number GTIN
          OP33-100UG 04055977208443
          OP33-20UG 07790788053901