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MABT404 Anti-hEF1/NEDD9, clone 2G9 Antibody

This Anti-hEF1/NEDD9, clone 2G9 Antibody is validated for use in Western Blotting for the detection of hEF1/NEDD9.

Anti-hEF1/NEDD9, clone 2G9 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: NEDD9, CASL, CASS2 UniProt Number: Q14511
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MABT404
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT404
      DescriptionAnti-hEF1/NEDD9, clone 2G9 Antibody
      Alternate Names
      • Enhancer of filamentation 1
      • hEF1
      • CRK-associated substrate-related protein
      • CAS-L
      • CasL
      • Cas scaffolding protein family member 2
      • Neural precursor cell expressed developmentally down-regulated protein 9
      • NEDD-9
      • Renal carcinoma antigen NY-REN-12
      • p105
      • Enhancer of filamentation 1 p55
      Background InformationEnhancer of filamentation 1, also known as CRK-associated substrate-related protein (CAS-L) or Cas scaffolding protein family member 2 or Neural precursor cell expressed developmentally down-regulated protein 9 (NEDD-9), or Renal carcinoma antigen NY-REN-12 p105, and encoded by the gene NEDD9 or CASL or CASS2, is a docking protein that plays a central role in coordinating tyrosine kinase mediated signaling for cell adhesion. NEDD9 controls the signals between focal adhesions at the cell periphery and the mitotic spindle in response to growth factors and signals. NEDD9 interacts with integrins and various other cellular proteins including BCAR3 and SH2D3C. It is localized to the cytoplasm in most cells but location can vary depending upon the cell type and function. NEDD9 is also found in cellular projections and lamellipodium and is found in the nucleus depending upon the cellular microenvironment. NEDD9 has multiple isoforms and active proteolytic fragments ranging from 55 kDa to 115 kDa. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with the mitotic spindle. NEDD9 is highly expressed in most cells and tissues and is typically induced upon cell growth and down regulated in adult tissues such as brain. NEDD9 is processed during the cell cycle into multiple proteolytic fragments that appear to have important functions at specific times during the cell cycle process. In cancer NEDD9 over expression can promote tumor growth and NEDD9 acts directly to stabilize Aurora A kinase which is often over expressed in many cancers. Destabilization of Aurora A via the inhibition or destruction of NEDD9 allows for tumor reduction, thus NEDD9 expression is being examined as a potential cancer marker.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-hEF1/NEDD9, clone 2G9 Antibody is validated for use in Western Blotting for the detection of hEF1/NEDD9.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected hEF1/NEDD9 in 10 µg of SH-SY5Y cell lysate.
      Biological Information
      ImmunogenRecombinant protein corresponding to human hEF1/NEDD9.
      Clone2G9
      Concentration1 mg/mL
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEDD9
      • CASL
      • CASS2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~93 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected hEF1/NEDD9 in 10 µg of MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT404 04053252977893

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies