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OP10L Anti-c-Myc (Ab-1) Mouse mAb (9E10)

This Anti-c-Myc (Ab-1) Mouse mAb (9E10) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Chromatin IP, Paraffin Sections for the detection of c-Myc (Ab-1).

Anti-c-Myc (Ab-1) Mouse mAb (9E10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP10L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Pricing & Availability

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OP10L-200UG
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      		 Plastic ampoule 200 μg
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      Description
      OverviewRecognizes a ~60-67 kDa c-Myc protein in HL60 cells and lung carcinoma tissue.
      Catalogue NumberOP10L
      Brand Family Calbiochem®
      References
      ReferencesLeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D. 1986. Ann. Rev. Gen. 20, 361.
      Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
      Nau, M.M., et al. 1985. Nature 318, 69.
      Persson, H., et al. 1984. Science 225, 687.
      Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Negative controlHT1080 cells
      Positive controlHL-60 cells or lung carcinoma
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesEpitope Tagging Munro, S., et al. 1986. Cell 46, 291. Immunoblotting Evan, G.I., et al. 1985. Mol. Cell. Biol. 5, 3610. Chromatin Immunoprecipitation Sanyal, K., et al. 2004. Proc. Natl. Acad. Sci. USA 101, 11374.
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Not Paraffin Sections
      Application NotesFlow Cytometry (20 µg/ml or use Cat. No. OP10F)
      Frozen Sections (2-10 µg/ml)
      Immunoblotting (1-5 µg/ml, see application references)
      Immunofluorescence (1-5 µg/ml or use Cat. No. OP10F)
      Immunoprecipitation (1 µg/sample)
      Chromatin Immunoprecipitation (5 µg/ml, see application references)
      Paraffin Sections (not recommended)
      Application CommentsThe c-Myc protein is extremely labile. c-Myc degradation can be as a result of freeze/thaw cycles, thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. This antibody recognizes the c-Myc protein and its cleavage products. The sequence of c-Myc predicts a ~45 kDa protein, but c-Myc migrates under reducing conditions as a ~64-67 kDa band. This antibody will not detect v-Myc but may react weakly to rodent c-Myc when used at high concentrations (10 µg/ml). An extra ~34-40 kDa band may be detected in a immunoblot. May also be used in immunofluorescence as well as the detection and purification of recombinant proteins tagged with the Myc epitope sequence EQKLISEEDL (see application references). The immunogen, c-Myc (Peptide-1) is also available for competition studies (Cat. No. PP06). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids 408-439 of human c-Myc
      ImmunogenHuman
      Epitopewithin amino acids 410-419
      Clone9E10
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP10L-200UG 04055977225044

      Documentation

      Anti-c-Myc (Ab-1) Mouse mAb (9E10) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-c-Myc (Ab-1) Mouse mAb (9E10) Certificates of Analysis

      TitleLot Number
      OP10L

      References

      Reference overview
      LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D. 1986. Ann. Rev. Gen. 20, 361.
      Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
      Nau, M.M., et al. 1985. Nature 318, 69.
      Persson, H., et al. 1984. Science 225, 687.
      Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      ApplicationFlow Cytometry (20 µg/ml or use Cat. No. OP10F)
      Frozen Sections (2-10 µg/ml)
      Immunoblotting (1-5 µg/ml, see application references)
      Immunofluorescence (1-5 µg/ml or use Cat. No. OP10F)
      Immunoprecipitation (1 µg/sample)
      Chromatin Immunoprecipitation (5 µg/ml, see application references)
      Paraffin Sections (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the ~64-67 kDa (apparent MW) c-Myc protein.
      BackgroundThe v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc, and B-myc. Amplification of the c-myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-myc gene has been found amplified in neuroblastomas, small cell lung carcinomas and Wilms tumors. L-myc is amplified in small cell lung cancer. Immunological studies have shown that the human c-myc gene gives rise to at least two nuclear phosphoproteins of ~64 kDa and ~67 kDa that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids 408-439 of human c-Myc
      Epitopewithin amino acids 410-419
      Clone9E10
      IsotypeIgG₁
      Specieshuman, mouse (weakly), rat (weakly)
      Positive controlHL-60 cells or lung carcinoma
      Negative controlHT1080 cells
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsThe c-Myc protein is extremely labile. c-Myc degradation can be as a result of freeze/thaw cycles, thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. This antibody recognizes the c-Myc protein and its cleavage products. The sequence of c-Myc predicts a ~45 kDa protein, but c-Myc migrates under reducing conditions as a ~64-67 kDa band. This antibody will not detect v-Myc but may react weakly to rodent c-Myc when used at high concentrations (10 µg/ml). An extra ~34-40 kDa band may be detected in a immunoblot. May also be used in immunofluorescence as well as the detection and purification of recombinant proteins tagged with the Myc epitope sequence EQKLISEEDL (see application references). The immunogen, c-Myc (Peptide-1) is also available for competition studies (Cat. No. PP06). Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
      Toxicity Standard Handling
      ReferencesLeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D. 1986. Ann. Rev. Gen. 20, 361.
      Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
      Nau, M.M., et al. 1985. Nature 318, 69.
      Persson, H., et al. 1984. Science 225, 687.
      Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
      Application referencesEpitope Tagging Munro, S., et al. 1986. Cell 46, 291. Immunoblotting Evan, G.I., et al. 1985. Mol. Cell. Biol. 5, 3610. Chromatin Immunoprecipitation Sanyal, K., et al. 2004. Proc. Natl. Acad. Sci. USA 101, 11374.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies