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ABE1342-100UG Anti-ZBTB4 (KAISO-L1)

Anti-ZBTB4 (KAISO-L1), Cat. No. ABE1342, is a highly specific rabbit polyclonal antibody that targets Zinc finger and BTB domain-containing protein 4 and has been tested for use in Immunohistochemistr

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Research Category: Epigenetics & Nuclear Function Gene Symbol: ZBTB4, KIAA1538 UniProt Number: Q9P1Z0
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ABE1342-100UG
100 μg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1342-100UG
      DescriptionAnti-ZBTB4 (KAISO-L1)
      Alternate Names
      • Zinc finger and BTB domain-containing protein 4
      • KAISO-like zinc finger protein 1
      • KAISO-L1
      Background InformationZinc finger and BTB domain-containing protein 4 (UniProt: Q9P1Z0; also known as KAISO-like zinc finger protein 1, KAISO-L1, ZBTB4) is encoded by the ZBTB4 (also known as KIAA1538) gene (Gene ID: 57659) in human. ZBTB4 is an epigenetic regulator that localizes at pericentromeric heterochromatin. It is shown to be an essential component in maintaining genomic stability in mammals. It serves as a transcriptional repressor with bimodal DNA-binding specificity. It represses transcription in a methyl-CpG-dependent manner and binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3', but can also bind to the non-methylated consensus sequence 5'-CTGCNA-3' (also known as the consensus kaiso binding site). ZBTB4 contains six C2H2-type zinc-finger domains and two proline-rich regions (aa 435-536 and 610-654). Its BTB (Broad-Complex, Tramtrack and Bric a brac) domain is localized in the amino acids 30-152. It is shown to be is frequently downregulated in cancer. ZBTB4 is phosphorylated by HIPK2 (Homeodomain Interacting Protein Kinase 2) and phosphorylation reduces its stability and triggers its degradation in response to DNA damage. (Ref.: Roussel-Gervais A., et al. (2017). Cancer Res; 77(1); 62-73; Yamada, D., et al (2009). Oncogene. 28(27); 2535-2544).
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-ZBTB4 (KAISO-L1), Cat. No. ABE1342, is a highly specific rabbit polyclonal antibody that targets Zinc finger and BTB domain-containing protein 4 and has been tested for use in Immunohistochemistry (Paraffin) and in Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected ZBTB4 (KAISO-L1) in human fallopian tube and human cervical cancer tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 17 amino acids from the internal region of human Zinc finger and BTB domain-containing protein 4.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Zinc finger and BTB domain-containing protein 4 (KAISO-L1) in human cells. It targets an epitope within 17 amino acids from the internal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Monkey, and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ZBTB4
      • KIAA1538
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~105 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC3 cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected ZBTB4 (KAISO-L1) in PC3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1342-100UG 04054839448133