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05-1946 Anti-USP7 Antibody, clone 1G7

This Anti-USP7 Antibody, clone 1G7 is validated for use in WB, IP, IH for the detection of USP7.

Anti-USP7 Antibody, clone 1G7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Ubiquitin & Ubiquitin Metabolism Gene Symbol: USP7, TEF1, HAUSP UniProt Number: Q93009
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05-1946
100 µg  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1946
      DescriptionAnti-USP7 Antibody, clone 1G7
      Alternate Names
      • ubiquitin specific peptidase 7 (herpes virus-associated)
      • Ubiquitin-specific-processing protease 7
      • ubiquitin specific protease 7 (herpes virus-associated)
      • Herpesvirus-associated ubiquitin-specific protease
      • Herpes virus-associated ubiquitin-specific protease
      • Ubiquitin thioesterase 7
      • ubiquitin specific peptidase 7
      • Deubiquitinating enzyme 7
      • HAUSP
      Background InformationUbiquitin carboxyl-terminal hydrolase 7 (USP7) is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates.[Saridakis, 2005] Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, Ubiquitin carboxyl-terminal hydrolase 7 activity generally stabilizes its substrate proteins. Ubiquitin carboxyl-terminal hydrolase 7 is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53.[Canning, 2004]
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-USP7 Antibody, clone 1G7 is validated for use in WB, IP, IH for the detection of USP7.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry
      Biological Information
      ImmunogenHistadine-tagged recombinant protein corresponding to human USP7.
      Clone1G7
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes USP7.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • USP7
      • TEF1
      • HAUSP
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCITON: Cleaves ubiquitin fusion protein substrates. Deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2, and also induces TP53-dependent cell growth repression and apoptosis.

      CATALYTIC ACTIVITY: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.

      ENZYME REGULATION: Inhibited by N-ethyl-maleimide (NEM) and divalent cations. Tolerates high concentrations of NaCl but is inhibited at concentrations of 195 mM and higher. Ref.7

      SUBUNIT STRUCTURE: Monomer. Interacts with TP53, MDM2 and UBXN6. Interacts with herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 and Epstein-Barr virus EBNA1. EBNA1 shows a 10-fold higher affinity than TP53 and can compete with it for USP7 binding. Binding to VMW110 may modulate the substrate specificity or activity of USP7 to stabilize viral proteins. Interacts with ATXN1 and the strength of interaction is influenced by the length of the poly-Gln region in ATXN1. A weaker interaction seen with mutants having longer poly-Gln regions.

      SUBCELLULAR LOCATION: Nucleus. Note: Present in a minority of ND10 nuclear bodies. Association with VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10. Colocalizes with ATXN1 in the nucleus.

      TISSUE SPECIFICTY: Widely expressed.

      PTM: Polyneddylated (By similarity).

      Polyubiquitinated (By similarity).

      SEQUENCE SIMILARITIES: Belongs to the peptidase C19 family.

      Contains 1 MATH domain.

      BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Active from pH 7.0 to 9.5.
      Molecular Weight~ 128kDa Observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected USP7 on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-1946 04053252628818

      Documentation

      Anti-USP7 Antibody, clone 1G7 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-USP7 Antibody, clone 1G7 Certificates of Analysis

      TitleLot Number
      Anti-USP7, clone 1G7 3049391
      Anti-USP7, clone 1G7 - 2142201 2142201
      Anti-USP7, clone 1G7 - 2145101 2145101
      Anti-USP7, clone 1G7 - 2167179 2167179
      Anti-USP7, clone 1G7 - 2436808 2436808
      Anti-USP7, clone 1G7 - 2227186 2227186
      Anti-USP7, clone 1G7 - 3215514 3215514
      Anti-USP7, clone 1G7 - 3394228 3394228
      Anti-USP7, clone 1G7 - 3588023 3588023
      Anti-USP7, clone 1G7 - 3836575 3836575
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies