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ABD29-25UG Anti-Twist1 (Twist-related protein 1) (rabbit polyclonal)

Detect Twist1 (Twist-related protein 1) using this Anti-Twist1 (Twist-related protein 1) Antibody validated for use in IH(P) & IC.

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ABD29-25UG
25 μg  
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      Description
      Catalogue NumberABD29-25UG
      DescriptionAnti-Twist1 (Twist-related protein 1) (rabbit polyclonal)
      Alternate Names
      • Twist-related protein 1
      • Class A basic helix-loop-helix protein 38
      • bHLHa38
      • H-twist
      Background InformationTwist1 is the basic helix-loop-helix transcription factor that regulates embryonic progenitor and transformed tumor cell proliferation, migration and differentiation and is also important in limb development. It has been shown that twist1 expression is high in patients suffering from rheumatic inflammatory disorders with chronically activated T helper lymphocytes and may be used as a biomarker for chronic t-cell activation. Mutations in twist1 are often associated with craniofacial and limb abnormalities in humans with Saethre-Chotzen syndrome. Twist1 dimer affinity can modify the DNA binding affinity. Twist1 has also been implicated as a promoter of tumor growth, invasion and anti-cancer-drug resistance.
      Product Information
      FormatAffinity Purified
      Control
      • Ductal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Twist1 (Twist-related protein 1) using this Anti-Twist1 (Twist-related protein 1) Antibody validated for use in IH(P) & IC.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected Twist1 in NIH/3T3, A431, and HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Twist1 near the N-terminus.
      EpitopeNear N-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis antibody recognizes Twist1 near the N-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Canine
      • Pig
      • Rhesus Macaque
      • Chimpanzee
      • Horse
      Species Reactivity NoteDemonstrated to react with Human and Mouse. Predicted to react with Pig, Rhesus Monkey, Chimpanzee, Equine, Gorilla, and Canine based on 100% sequence homology. Other homologies: Rat (92% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryBasic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq].
      Gene Symbol
      • TWIST1
      • BHLHA38
      • TWIST
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.

      SUBUNIT STRUCTURE: Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed (By similarity).

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICTY: Subset of mesodermal cells.

      INVOLVEMENT IN DISEASE: Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.

      Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.

      Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.

      SEQUENCE SIMILARITIES: Contains 1 basic helix-loop-helix (bHLH) domain.
      Molecular Weight21 kDa calculated
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in ductal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues.

      Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Twist1 in ductal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Global Trade Item Number
      Catalogue Number GTIN
      ABD29-25UG 04054839342585