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ABN2178-25UL Anti-Tau (TauC4 Antibody, 354-369)

Anti-Tau (TauC4, 354-369), Cat. No. ABN2178, is a highly specific rabbit polyclonal antibody that targets TauC4 region of Tau and has been tested for use in Electron Microscopy and Western Blotting.

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ABN2178-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABN2178-25UL
      DescriptionAnti-Tau (TauC4 Antibody, 354-369)
      Alternate Names
      • Microtubule-associated protein tau
      • Neurofibrillary tangle protein
      • Paired helical filament-tau
      • PHF-tau
      Background InformationMicrotubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human. Microtubule-associated protein tau is shown to be expressed in neurons. It is mostly found in axons, in the cytosol, and in association with plasma membrane components. Nine isoforms of Tau have been described that are generated by alternative splicing. Tau proteins promote microtubule assembly and stability and may also be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, thus serving as a linker protein between both. The short isoforms are reported to allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. In Alzheimer disease (AD), the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of Tau. In AD brain Tau accumulates in both the somatodendritic and axonal domains of neurons and it also accumulates in the soma as neurofibrillary tangles (NFTs). Tau oligomers are reported to be neurotoxic when applied extracellularly to cultured neuronal cells and can induce neurodegeneration and synaptic and mitochondrial dysfunction in vivo. It has been reported that most of the C-terminus of Tau (aa 243-406) in PHFs can be cleaved by trypsin, however the TauC4 region is highly resistant to trypsin action. (Ref.: Taniguchi Watanabe, S., et al. (2016). Acta Neuropathol. 131(2); 267-280; Ando K., et al. (2010). Biochem. Soc. Trans. 38(4); 1001-1005).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antiserum with 0.1% sodium azide.
      Applications
      ApplicationAnti-Tau (TauC4, 354-369), Cat. No. ABN2178, is a highly specific rabbit polyclonal antibody that targets TauC4 region of Tau and has been tested for use in Electron Microscopy and Western Blotting.
      Key Applications
      • Electron Microscopy
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Tau (TauC4, 354-369) in Western Blotting applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190; Taniguchi-Watanabe, S., et. al. (2016). Acta Neuropathol. 131(2):267-280).

      Electron Microscopy Analysis: A representative lot detected Tau (TauC4, 354-369) in Immunogold negative-stain Electron Microscopy applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Microtubule-associated protein tau, isoform 8 (Tau-F).
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects TauC4 region in human Tau protein.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MAPT
      • MAPTL
      • MTBT1
      • TAU
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~55 kDa observed; 45.85 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Recombinant full-length 4-repeat (4R) tau (human 4R1N).

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected TauC4 region in full length recombinant human tau (human 4R1N).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN2178-25UL 04054839636639