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MABN819 Anti-Tau Antibody, oligomeric Antibody, clone TOMA-1

Anti-Tau, oligomeric, clone TOMA-1, Cat. No. MABN819, is amouse monoclonal antibody that targets tau oligomers and has been tested in Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry, Neutralization, and Western Blotting applications.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: MAPT, TAU, MTBT1, MAPTL UniProt Number: P10636
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MABN819
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RDB, ELISA, IF, IHC, Neutralization Assay, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN819
      DescriptionAnti-Tau Antibody, oligomeric Antibody, clone TOMA-1
      Alternate Names
      • Microtubule-associated protein tau oligomer
      • Tau oligomer
      • PHF-tau oligomer
      • Paired helical filament-tau oligomer
      • Neurofibrillary tangle protein oligomer
      Background InformationMicrotubule-associated protein tau (UniProt P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as TAU, MAPT1, MTBTL) gene (Gene ID 4137) in human. In Alzheimer's disease (AD) pathology, accumulation of the microtubule-associated protein tau takes place primarily in the neurons. Tau accumulates in both the somatodendritic and axonal domains of neurons. Tau also accumulates in the soma as neurofibrillary tangles (NFTs). Cell death and synaptic lesions occur independently of NFT formation, and research indicates that NFT formation alone is insufficient for neurodegeneration, suggesting that soluble tau aggregates may be the more toxic and pathologically significant tau species. Tau oligomers are neurotoxic when applied extracellularly to cultured neuronal cells, and tau oligomers (but not fibrils) induce neurodegeneration and synaptic and mitochondrial dysfunction in vivo. Moreove, researchers are able to use tau oligomers as a reliable biomarker to differentiate AD brains from age-matched non-AD brains.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2aκ in buffer containing PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Tau, oligomeric, clone TOMA-1, Cat. No. MABN819, is amouse monoclonal antibody that targets tau oligomers and has been tested in Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry, Neutralization, and Western Blotting applications.
      Key Applications
      • Dot Blot
      • ELISA
      • Immunofluorescence
      • Immunohistochemistry
      • Neutralization Assay
      • Western Blotting
      Application NotesWestern Blotting Analysis: 20 µg/mL from a representative lot detected upregulated oligomeric tau in soluble PBS brain extract from Alzheimer's diseased (AD) human brain (Courtesy of Sengupta, U., et al., Kayed lab, University of Texas, Galveston).

      Please refer to the following publications regarding the use of TOMA clones in Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry, Neutralization, and Western Blotting applications:

      1. Vuono, R., et al. (2015). Brain. 138(Pt 7):1907-1918.
      2. Castillo-Carranza, D.L., et al. (2015). J. Neurosci. 35(12):4857-4868.
      3. Castillo-Carranza, D.L., et al. (2014). J. Neurosci. 34(12):4260-4272.
      Biological Information
      ImmunogenRecombinant human Tau-441 (Tau-F, Tau-4, 2N4R isoform) oligomers (Lasagna-Reeves, C. A., et al. (2012). FASEB J. 26(5):1946-1959).
      CloneTOMA-1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone TOMA-1 (a.k.a. clone H12C10) is among more than 13 hybridomas derived from mice immunized with in vitro generated recombinant Tau-441 (Tau-F, Tau-4, 2N4R isoform) oligomers. These TOMAs are reported to specifically detect oligomeric tau without any significant reactivity toward monomeric tau, tau fibrils, Aβ oligomers, Aβ fibrils, or α-synuclein oligomers (Castillo-Carranza, D.L., et al. (2014). J. Neurosci. 34(12):4260-4272).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Mammals based on high homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MAPT
      • TAU
      • MTBT1
      • MAPTL
      Purification MethodProtein A purified.
      UniProt Number
      Molecular WeightVariable depending on the size(s) of the oligomer(s). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Alzheimer's diseased human brain lysate.

      Western Blotting Analysis: 4 µg/mL of this antibody detected Tau oligmers in 25 µg of Alzheimer's diseased human brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN819 04054839081415

      Documentation

      Anti-Tau Antibody, oligomeric Antibody, clone TOMA-1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tau Antibody, oligomeric Antibody, clone TOMA-1 Certificates of Analysis

      TitleLot Number
      Anti-Tau, oligomeric, clone TOMA-1 - 3185631 3185631
      Anti-Tau, oligomeric, clone TOMA-1 - 3327591 3327591
      Anti-Tau, oligomeric, clone TOMA-1 - 3538093 3538093
      Anti-Tau, oligomeric, clone TOMA-1 - 3700354 3700354
      Anti-Tau, oligomeric, clone TOMA-1 - 3905434 3905434
      Anti-Tau, oligomeric, clone TOMA-1 - 4020935 4020935
      Anti-Tau, oligomeric, clone TOMA-1 - 4068641 4068641
      Anti-Tau, oligomeric, clone TOMA-1 - 4164248 4164248
      Anti-Tau, oligomeric, clone TOMA-1 -Q2767837 Q2767837
      Anti-Tau, oligomeric, clone TOMA-1 Monoclonal Antibody 3017561
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