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MABF2156-100UL Sigma-AldrichAnti-TLR9 Antibody, clone NaR9

Anti-TLR9, clone NaR9, Cat. No. MABF2156, is a mouse monoclonal antibody that detects Toll-like receptor 9 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Inhibition/ Function Analysis.

MABF2156-100UL

100 µL

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Overview

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Description
Catalogue Number	MABF2156-100UL
Description	Anti-TLR9 Antibody, clone NaR9
Alternate Names	Toll-like receptor 9 CD289
Background Information	Toll-like receptor 9 (UniProt: Q9EQU3; also known as CD289) is encoded by the Tlr9 gene (Gene ID: 81897) in murine species. TLR9 is a single-pass type I membrane protein that serves as an innate immune sensor that recognizes microbial DNA. It exists as a monomer in the absence of unmethylated cytidine-phosphate-guanosine (CpG) ligand. TLR9 is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to generate the mature form. The mature form contains an extracellular domain (aa 26-818), a short transmembrane domain (aa 819-839), and the cytoplasmic domain (aa 840-1032). It is expressed in the basolateral region of gastric epithelial cells and high levels are detected in antrum and body mucosa. Ligand recognition of TLR9 is shown to be strictly controlled to prevent unnecessary response to self-derived DNA such as mitochondrial DNA. Ligand-dependent TLR9 trafficking plays a critical role in type I interferon induction in macrophages and plasmacytoid dendritic cells. TLR9 is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides and acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion, and the inflammatory response. (Ref.: Murakami, Y., et al. (2017). Sci. Rep. 7; 44042).

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal antibody IgG2a in PBS without azide.

Applications
Application	Anti-TLR9, clone NaR9, Cat. No. MABF2156, is a mouse monoclonal antibody that detects Toll-like receptor 9 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Inhibition/ Function Analysis.
Key Applications	Flow Cytometry Immunocytochemistry Inhibits Activity/Function
Application Notes	Immunocytochemistry Analysis: A representative lot detected TLR9 in Immunocytochemistry applications (Murakami, Y., et. al. (2017). Sci Rep. 7:44042). Flow Cytometry Analysis: A representative lot detected TLR9 in Flow Cytometry applications (Murakami, Y., et. al. (2017). Sci Rep. 7:44042). Inhibits Activity/Function: A representative lot inhibited TLR9-dependent cytokine production in vitro by bone marrow-derived macrophages and conventional dendritic cells. (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).

Biological Information
Immunogen	Ba/F3 cells expressing full-length mouse TLR9 protein. Immunization was carried out on Tlr9 -/- mice.
Clone	NaR9
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone NaR9 is a mouse monoclonal antibody that specifically detects Toll-like receptor 9 in murine species. It targets an epitope within the N-terminal region.
Isotype	IgG2aκ
Species Reactivity	Mouse
Species Reactivity Note	Mouse.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_112455.2
Gene Symbol	Tlr9
Purification Method	Protein G purified
UniProt Number	Q9EQU3
Molecular Weight	116.41 kDa calculated.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Immunocytochemistry in Raw264.7 cells. Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected TLR9 in Raw264.7 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
MABF2156-100UL	04061841030478

Documentation

Anti-TLR9 Antibody, clone NaR9 SDS

Title
Safety Data Sheet (SDS)

Anti-TLR9 Antibody, clone NaR9 Certificates of Analysis

Title	Lot Number
Anti-TLR9, clone NaR9 - 3756548	3756548
Anti-TLR9, clone NaR9 - 4050739	4050739
Anti-TLR9, clone NaR9 - 4113815	4113815
Anti-TLR9, clone NaR9 - Q3269852	Q3269852

References

Reference overview	Pub Med ID
The protective effect of the anti-Toll-like receptor 9 antibody against acute cytokine storm caused by immunostimulatory DNA. Murakami, Y; Fukui, R; Motoi, Y; Shibata, T; Saitoh, SI; Sato, R; Miyake, K Sci Rep 7 44042 2017 Show Abstract Toll-like Receptor 9 (TLR9) is an innate immune receptor recognizing microbial DNA. TLR9 is also activated by self-derived DNA, such as mitochondrial DNA, in a variety of inflammatory diseases. We show here that TLR9 activation in vivo is controlled by an anti-TLR9 monoclonal Ab (mAb). A newly established mAb, named NaR9, clearly detects endogenous TLR9 expressed in primary immune cells. The mAb inhibited TLR9-dependent cytokine production in vitro by bone marrow-derived macrophages and conventional dendritic cells. Furthermore, NaR9 treatment rescued mice from fulminant hepatitis caused by administering the TLR9 ligand CpGB and D-(+)-galactosamine. The production of proinflammatory cytokines induced by CpGB and D-(+)-galactosamine was significantly impaired by the mAb. These results suggest that a mAb is a promising tool for therapeutic intervention in TLR9-dependent inflammatory diseases.	28266597

Reference overview

Pub Med ID

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