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07-2167 Anti-SUMO 2/3 Antibody

This SUMO 2/3 antibody is validated for use in WB & IP for the detection of the SUMO 2/3 protein.

Anti-SUMO 2/3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Ubiquitin & Ubiquitin Metabolism Gene Symbol: SMT3B, SMT3H2, SUMO3, HSMT, MGC117191, sentrin 2, Sentrin-2, SUMO-3, SUMO-2 UniProt Number: P61956
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07-2167
100 µg  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, Chp, Po, Rhesus Macaque, OpWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2167
      DescriptionAnti-SUMO 2/3 Antibody
      Alternate Names
      • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
      • Ubiquitin-like protein SMT3B
      • SMT3 homolog 2
      • SMT3 (suppressor of mif two 3, yeast) homolog 2
      • SMT3 suppressor of mif two 3 homolog 2 (yeast)
      • small ubiquitin-related modifier 2
      • small ubiquitin-like modifier 2
      Background InformationSmall ubiquitin-related modifier 2 (UniProt P61956; also known as HSMT3, Sentrin-2, SMT3 homolog 2, Smt3B, SUMO-2, Ubiquitin-like protein SMT3B) and 3 (UniProt P55854; also known as SMT3 homolog 1, Smt3A, SUMO-3, Ubiquitin-like protein SMT3A) are encoded by the SUMO2 (also known as SMT3B, SMT3H2; Gene ID 6613) and SUMO3 (also known as SMT3A, SMT3H1; Gene ID 6612) genes in human. SUMOylation, protein post-translation modification by small ubiquitin-like modifier (SUMO), is a signaling event in many cellular processes. SUMO proteins are translated as immature precursors and subsequently converted to their mature forms through the activity of sentrin/SUMO-specific proteases (SENPs). SUMOylation is a reversible process. SUMO E1 activating enzyme, E2 conjugating enzyme, and E3 ligase mediate SUMOylation of substrate proteins, while SENPs are responsible for the de-SUMOylation. SUMOylation usually occurs at lysine residues in the consensus KxD/E motif, although not all such lysines become SUMOylated and SUMOylation can also occur on lysine residues outside of this motif. SUMO2 and 3 share 97% identity at the amino acid level, while SUMO1 shares only about 50% sequence homology with SUMO-2 and 3. In addition to difference in their target substrates, SUMO2/3 can be SUMOylated and form chains, whereas SUMO1 cannot and may serve as chain terminator. SUMO-2 can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Its covalent attachment to its substrate via an isopeptide bond requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Although SUMO-2 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system, however, unlike ubiquitin which targets proteins for degradation, SUMO-2 is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus (aa 94-95; propeptide) have been cleaved off.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa nuclear extract
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 without azide.
      Quality LevelMQ100
      Applications
      ApplicationThis SUMO 2/3 antibody is validated for use in WB & IP for the detection of the SUMO 2/3 protein.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected SUMO 2/3 on 10 µg of HeLa nuclear extract.
      Immunoprecipitation Analysis: A previous was used by an independent laboratory in IP. (Li, T., et al. (2006). The Journal of Biological Chemistry. 281(47):36221-36227.)
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the first 15 amino acids from the N-terminal region of human SUMO-2/3.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes SUMO 2/3 at the N-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Chimpanzee
      • Pig
      • Rhesus Macaque
      • Opossum
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Predicted to react with bovine, rhesus macaque, porcine, opossum, and chimpanzee based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • SMT3B
      • SMT3H2
      • SUMO3
      • HSMT
      • MGC117191
      • sentrin 2
      • Sentrin-2
      • SUMO-3
      • SUMO-2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.
      SUBUNIT STRUCTURE: Homotrimer Potential. Crystal packing analysis suggests a possible trimeric assembly, of which the biological significance remains to be determined. Interacts with SAE2 and UBE2I. Covalently attached to a number of proteins. Interacts with PELP1. Interacts with USP25; the interaction sumoylates USP25.
      SUBCELLULAR LOCATION: Nucleus. Note: Nuclear bodies.
      TISSUE SPECIFICITY: Broadly expressed.
      PTM: Polymeric chains can be formed through Lys-11 cross-linking. Polymeric SUMO2 chains undergo 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4.
      Cleavage of precursor form by SENP1 or SENP2 is necessary for function.
      SEQUENCE SIMILARITIES: Belongs to the ubiquitin family. SUMO subfamily.
      Contains 1 ubiquitin-like domain.
      Molecular Weight~12 kDa observed; 10.87 for SUMO-2 and 11.64 for SUMO-3 calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.
      Western Blot Analysis: 1 µg/mL of this antibody detected SUMO 2/3 on 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-2167 04053252641565