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MABT213 Anti-SHP-1 Antibody, clone HG213, rabbit monoclonal

Anti-SHP-1 Antibody, clone HG213 detects level of SHP-1 & has been published & validated for use in WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: PTPN6, HCP, PTP1C UniProt Number: P29350
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MABT213
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbUnconjugatedMonoclonal Antibody
      Description
      Catalogue NumberMABT213
      DescriptionAnti-SHP-1 Antibody, clone HG213, rabbit monoclonal
      Alternate Names
      • Tyrosine-protein phosphatase non-receptor type 6
      • Hematopoietic cell protein-tyrosine phosphatase
      • Protein-tyrosine phosphatase 1C
      • PTP-1C
      • Protein-tyrosine phosphatase SHP-1
      • SH-PTP1
      Background InformationSrc Homology region 2 domain Phosphatase 1 (SHP-1) is also known as Protein Tyrosine Phosphatase 1C (PTP1C), Protein Tyrosine Phosphatase non-receptor type 6 (PTPN6), and Hematopoietic Cell Phosphatase (HCPH). SHP-1 is a SH2 domain-containing cytosolic tyrosine phosphatase characterized by a catalytic phosphatase domain of approximately 240 amino acids that selectively dephosphorylates tyrosine residues in proteins; the SH2 domains are critical for SHP-1 to bind to ITIM domains in inhibitory immunoreceptors. SHP-1 is highly expressed in hematopoietic tissues and has a negative impact on lymphocyte signaling. SHP-1 is implicated in the control of tyrosine kinase signaling pathways in cellular proliferation, with a potential role in cancer.
      References
      Product Information
      FormatUnconjugated
      Control
      • Jurkat cell lysate
      PresentationRabbit monoclonal supernatant without sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SHP-1 Antibody, clone HG213 detects level of SHP-1 & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenFull-length glutathione transferase SHP-1 fusion protein, purified from bacteria.
      CloneHG213
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.
      Gene Symbol
      • PTPN6
      • HCP
      • PTP1C
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.

      SIZE: 595 amino acids; 67561 Da

      SUBUNIT: Monomer. Interacts with MTUS1 (By similarity). Binds PTPNS1, LILRB1 and LILRB2. Interacts with FCRL2, CD300LF and CD84.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus (By similarity). Note=In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity).

      TISSUE SPECIFICITY: Isoform 1 is expressed in hematopoietic cells while isoform 2 is expressed in non-hematopoietic cells.

      PTM: Phosphorylated on serine and tyrosine residues.

      SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily. & Contains 2 SH2 domains. & Contains 1 tyrosine-protein phosphatase domain.
      Molecular Weight~67 kDa observed.
      3 isoforms exist with molecular weights between 63 kDa and 68 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Jurkat cell lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected SHP-1 in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT213 04053252535598

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies