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MABF1995-25UL Sigma-AldrichAnti-PvRBP2b Antibody, clone 3E9

Anti-PvRBP2b, clone 3E9, Cat. No. MABF1995, is a mouse monoclonal antibody that detects Reticulocyte-binding protein 2 in Plasmodium vivax and has been tested for use in ELISA, Flow Cytometry, Inhibition/ Function Assay, and Western Blotting.

MABF1995-25UL

25 μg

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Overview

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Description
Catalogue Number	MABF1995-25UL
Description	Anti-PvRBP2b Antibody, clone 3E9
Alternate Names	Reticulocyte-binding protein 2
Background Information	Reticulocyte-binding protein 2 (UniProt: Q00799; also known as PvRBP2) is encoded by the RBP-2 (also known as RBP2) gene in Plasmodium species. PvRBP2b is an alpha-helical protein, comprising 10 alpha-helices and two very short antiparallel beta-sheets, each comprising two beta-strands. It plays a critical role in reticulocyte recognition and invasion by Plasmodium vivax. It is synthesized with a signal peptide (aa 1-21), a large extracellular domain (aa 22-2805), a short transmembrane domain (aa 2806-2826), and a cytoplasmic tail (aa 2827-2867). Plasmodium vivax is shown to preferentially invade reticulocytes that express high levels of transferrin receptor 1 (TfR1). PvRBP2b is expressed during the late-stage of Plasmodium vivax and its binding has been shown to directly correlate with the levels of TfR1 on reticulocyte surface. Treatment of reticulocytes with trypsin or chymotrypsin can cleave TfR1 from reticulocyte surface and prevent PvRBP2b binding. Clone 3E9 is shown to inhibit reticulocyte binding and block the entry of Plasmodium vivax into reticulocytes. This antibody binds to the side of the N-terminal domain of PvRBP2b to a region localized between helices alpha 4, 5 and 6. (Ref.: Gruszczyk, J., et al. (2018). Science 359(6371); 48-55; Gruszczyk, J., et al. (2018). Nature. 559(7712); 135-139).

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal antibody IgG2a in PBS without azide.

Applications
Application	Anti-PvRBP2b, clone 3E9, Cat. No. MABF1995, is a mouse monoclonal antibody that detects Reticulocyte-binding protein 2 in Plasmodium vivax and has been tested for use in ELISA, Flow Cytometry, Inhibition/ Function Assay, and Western Blotting.
Key Applications	ELISA Flow Cytometry Inhibits Activity/Function Western Blotting
Application Notes	Inhibits Activity/Function Analysis: A representative lot inhibited PvRBP2b binding to reticulocytes and blocked the invasion of P. vivax into reticulocytes. (Gruszczyk, J., et. al. (2018). Science. 359(6371):48-55). Flow Cytometry Analysis: A representative lot detected PvRBP2b in Flow Cytometry applications (Gruszczyk, J., et. al. (2018). Science. 359(6371):48-55). Enzyme Immunoassay Analysis: A representative lot detected PvRBP2b in ELISA applications (Gruszczyk, J., et. al. (2018). Science. 359(6371):48-55). .

Biological Information
Immunogen	His-tagged recombinant fragment corresponding to 1294 amino acids from the N-terminal half of Plasmodium vivax Reticulocyte-binding protein 2 (PvRBP2b).
Epitope	extracellular domain
Clone	3E9
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone 3E9 is a mouse monoclonal antibody that specifically detects Reticulocyte binding protein 2b (PvRBP2b) in Plasmodium vivax.
Isotype	IgG2aκ
Species Reactivity	Parasite
Species Reactivity Note	Plasmodium vivax.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	na
Gene Symbol	RBP-2 RBP2
Purification Method	Protein G purified
UniProt Number	Q00799
Molecular Weight	~120-150 kDa observed; 331.44 kDa calculated (for full length 2pVRBP2b). Uncharacterized bands may be observed in some lysate(s).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in Plasmodium vivax under non-reducing conditions. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected purified Reticulocyte-binding protein from Plasmodium vivax (2PvRBP2b) under non-reducing conditions.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	25 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
MABF1995-25UL	04054839997235

Documentation

Anti-PvRBP2b Antibody, clone 3E9 SDS

Title
Safety Data Sheet (SDS)

Anti-PvRBP2b Antibody, clone 3E9 Certificates of Analysis

Title	Lot Number
Anti-PvRBP2b, clone 3E9 - 3238884	3238884

References

Reference overview	Pub Med ID
Transferrin receptor 1 is a reticulocyte-specific receptor for Plasmodium vivax. Gruszczyk, J; Kanjee, U; Chan, LJ; Menant, S; Malleret, B; Lim, NTY; Schmidt, CQ; Mok, YF; Lin, KM; Pearson, RD; Rangel, G; Smith, BJ; Call, MJ; Weekes, MP; Griffin, MDW; Murphy, JM; Abraham, J; Sriprawat, K; Menezes, MJ; Ferreira, MU; Russell, B; Renia, L; Duraisingh, MT; Tham, WH Science 359 48-55 2018 Show Abstract Plasmodium vivax shows a strict host tropism for reticulocytes. We identified transferrin receptor 1 (TfR1) as the receptor for P. vivax reticulocyte-binding protein 2b (PvRBP2b). We determined the structure of the N-terminal domain of PvRBP2b involved in red blood cell binding, elucidating the molecular basis for TfR1 recognition. We validated TfR1 as the biological target of PvRBP2b engagement by means of TfR1 expression knockdown analysis. TfR1 mutant cells deficient in PvRBP2b binding were refractory to invasion of P. vivax but not to invasion of P. falciparum Using Brazilian and Thai clinical isolates, we show that PvRBP2b monoclonal antibodies that inhibit reticulocyte binding also block P. vivax entry into reticulocytes. These data show that TfR1-PvRBP2b invasion pathway is critical for the recognition of reticulocytes during P. vivax invasion.	29302006

Reference overview

Pub Med ID

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