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16-282 Anti-Phosphotyrosine Antibody, clone 4G10®, Magnetic Bead Conjugate

Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10®, Magnetic Bead Conjugate validated for use in IP.

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Research Category: Signaling Research Sub-Category: General Post-translation Modification
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16-282
200 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AIPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number16-282
      DescriptionAnti-Phosphotyrosine Antibody, clone 4G10®, Magnetic Bead Conjugate
      Background InformationTyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.).The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
      Product Information
      FormatPurified
      Control
      • NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
      Presentation20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 IPs.
      Quality LevelMQ100
      Applications
      ApplicationDetect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10®, Magnetic Bead Conjugate validated for use in IP.
      Key Applications
      • Immunoprecipitation
      Biological Information
      ImmunogenPhosphotyramine-KLH
      Cloneclone 4G10®
      HostMouse
      SpecificityThis antibody recognizes tyrosine-phosphorylated proteins from all species
      IsotypeIgG2bκ
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G-Sepharose™ Chromatography
      Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
      Product Usage Statements
      Quality Assurance100 μg of NIH3T3 whole cell lysate stimulated with 100 ng/mL PDGF (10 minutes) was immunoprecipitated (IP) using either anti-mouse IgG (Cat. No. 12-371) (lane 1), or Anti-Phosphotyrosine, Magnetic Bead Conjugate
      (Lane 2).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Note: DO NOT FREEZE
      Packaging Information
      Material Size200 µL
      Global Trade Item Number
      Catalogue Number GTIN
      16-282 04053252641596