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MABT1441-100UG Anti-α-Parvin Antibody, clone 1D4

Anti-a-Parvin, clone 1D4 , Cat. No. MABT1441, is a mouse monoclonal antibody that detects a-Parvin and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

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MABT1441-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1441-100UG
      DescriptionAnti-α-Parvin Antibody, clone 1D4
      Alternate Names
      • Actopaxin
      • CH-ILKBP
      • Calponin-like integrin-linked kinase-binding protein
      • Matrix-remodeling-associated protein 2
      Background InformationAlpha-Parvin (UniProt: Q9NVD7; also known as Actopaxin, CH-ILKBP, Calponin-like integrin-linked kinase-binding protein, Matrix-remodeling-associated protein 2) is encoded by the PARVA (also known as MXRA2) gene (Gene ID: 55742) in human. a-Parvin is a widely expressed, highly conserved, peripheral membrane protein that is highly expressed in heart, skeletal muscle, kidney, and liver. It contains two calponin-homology (CH1 and CH2) domains that are localized in amino acids 95-202 and 262-369. It interacts with the carboxy terminal of integrin-linked kinase (ILK) via its CH2 domain and deletion of its CH2 domain is reported to abolish its ability to localize to focal adhesions. a-Parvin plays a role in sarcomere organization and in smooth muscle cell contraction and is also required for normal development of the embryonic cardiovascular system. It is also essential for normal septation of the heart outflow tract. a-Parvin also plays a role in sprouting angiogenesis and is required for normal adhesion of vascular smooth muscle cells to endothelial cells during blood vessel development. It is also reported to play a role in establishing cell polarity, reorganization of the actin cytoskeleton, formation of lamellipodia and ciliogenesis. (Ref.: Tu, Y., et al. (2001). J. Cell Biol. 153(3); 585-598).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-a-Parvin, clone 1D4 , Cat. No. MABT1441, is a mouse monoclonal antibody that detects a-Parvin and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • ELISA
      • Western Blotting
      • Immunoprecipitation
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected a-Parvin in Western Blotting applications (Tu, Y., et al. (2001). J Cell Biol. 153(3):585-98).

      Immunofluorescence Analysis: A representative lot detected a-Parvin in Immunofluorescsence applications (Tu, Y., et al. (2001). J Cell Biol. 153(3):585-98).

      ELISA Analysis: A representative lot detected a-Parvin in ELISA applications (Tu, Y., et al. (2001). J Cell Biol. 153(3):585-98).

      Immunoprecipitation Analysis: A representative lot detected a-Parvin in Immunoprecipitation applications (Tu, Y., et al. (2001). J Cell Biol. 153(3):585-98).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 351 amino acids from human a-Parvin.
      EpitopeC-terminal half
      Clone1D4
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1D4 is a mouse monoclonal antibody that detects a-Parvin. It targets an epitope within 29 amino acids from the C-terminal half.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Hamster based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PARVA
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight42.24 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in C2C12 cell line.

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected a-Parvin in C2C12 cell line.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1441-100UG 04065267780535