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454578 Anti-Pan-NMDA NR1 Mouse mAb (R1JHL)

This Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) is validated for use in Immunoblotting for the detection of Pan-NMDA NR1.

Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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454578
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
RMMonoclonal Antibody

Pricing & Availability

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454578-15UG
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      		 Plastic ampoule 15 μg
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      Description
      OverviewRecognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts.
      Catalogue Number454578
      Brand Family Calbiochem®
      References
      ReferencesLaurie, D.J., and Seeburg, P.H. 1994. J. Neurosci. 14, 3180
      Ishii, T., et al. 1993. J. Biol. Chem. 268, 2836.
      Monyer, H., et al. 1992. Science 256, 1217.
      Product Information
      FormLyophilized
      FormulationLyophilized from cell culture supernatant.
      Positive controlRat cortex tissue extracts
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:300, colorimetric)
      Immunoprecipitation (3 µg/200 µg lysate)
      Application CommentsDoes not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1
      ImmunogenRat
      CloneR1JHL
      HostMouse
      IsotypeIgG
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      454578-15UG 04055977204155

      Documentation

      Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) Certificates of Analysis

      TitleLot Number
      454578

      References

      Reference overview
      Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci. 14, 3180
      Ishii, T., et al. 1993. J. Biol. Chem. 268, 2836.
      Monyer, H., et al. 1992. Science 256, 1217.

      Brochure

      Title
      Pathways and Biomarkers of Glutamatergic Synapse Flyer
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-August-2008 JSW
      ApplicationImmunoblotting (1:300, colorimetric)
      Immunoprecipitation (3 µg/200 µg lysate)
      DescriptionMouse monoclonal antibody lyophilized from cell culture supernatant. Recognizes the ~115 kDa subunit of the NMDAR (N-methyl-D-aspartate receptor) protein.
      BackgroundThe rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. NR1 can form NMDA-activated channels when expressed in oocytes, but the currents are larger when combined with one or more of the NMDAR2 (NR2 A-D) subunits.
      HostMouse
      Immunogen speciesRat
      Immunogena recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1
      CloneR1JHL
      IsotypeIgG
      Speciesrat
      Positive controlRat cortex tissue extracts
      FormLyophilized
      FormulationLyophilized from cell culture supernatant.
      PreservativeNone
      CommentsDoes not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesLaurie, D.J., and Seeburg, P.H. 1994. J. Neurosci. 14, 3180
      Ishii, T., et al. 1993. J. Biol. Chem. 268, 2836.
      Monyer, H., et al. 1992. Science 256, 1217.