Millipore Sigma Vibrant Logo

MABS1978 Anti-Osteocalcin (Glu17) Antibody, clone 5C5

Anti-Osteocalcin (Glu17), clone 5C5, Cat. No. MABS1978, is a mouse monoclonal antibody that detects decarboxylated osteocalcin and has been tested for use in Dot Blot and Enzyme Immunoassay (ELISA).

Anti-Osteocalcin (Glu17) Antibody, clone 5C5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: BGLAP UniProt Number: P02818
View Products on Sigmaaldrich.com
MABS1978
100 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Fulfillment and delivery delayed Fulfillment and delivery delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HDB, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1978
      DescriptionAnti-Osteocalcin (Glu17) Antibody, clone 5C5
      Alternate Names
      • Bone Gla protein
      • BGP
      • Gamma-carboxyglutamic acid-containing protein
      Background InformationOsteocalcin (UniProt: P02818; also known as Bone Gla protein, BGP, Gamma-carboxyglutamic acid-containing protein) is encoded by the BGLAP gene (Gene ID: 632) in human. Osteocalcin is a member of the osteocalcin/matrix Gla protein family and can constitute 1 to 2% of the total bone protein. It is synthesized by osteoblasts and is a major component of the noncollagenous bone matrix. Osteocalcin is synthesized with a signal peptide (aa 1-23), a propetide (aa 24-51) that are cleaved to generate active secreted form of osteocalcin (aa 52-100). Osteocalcin displays a high affinity for calcium and binds strongly to apatite. Sixty to ninety percent of de novo synthesized osteocalcin is incorporated into the bone matrix where it binds to hydroxyapatite during matrix mineralization. The remainder of the osteocalcin is released into the circulation where it can be measured as a sensitive marker of bone formation. Osteocalcin found in serum is almost exclusively derived from the bone formation with little or no contribution from the resorption process. A major feature of osteocalcin is the presence of 3 vitamin K-dependent gamma-carboxyglutamic acid (Gla) residues, which are found in the first helical region and interact with the inter-calcium spacing in the HAP lattice. In humans, the percentage of the circulating osteocalcin that is not gamma-carboxylated is used as a biomarker of vitamin K status. Osteocalcin expression in osteoblasts is stimulated by insulin and adiponectin and osteocalcin enhances insulin sensitivity and differentiation in muscle. Serum osteocalcin is elevated in diseases characterized by increased bone turnover such as osteoporosis, hyperparathyroidism and Paget's disease, and low in conditions associated with low bone turnover such as hypoparathyroidism and growth hormone deficiency. (Ref.: Kanazawa I (2015). World J Diabetes. 6(18): 1345 1354; Gundberg, CM et al. (2012). Adv Nutr 3 (2): 149-157).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Osteocalcin (Glu17), clone 5C5, Cat. No. MABS1978, is a mouse monoclonal antibody that detects decarboxylated osteocalcin and has been tested for use in Dot Blot and Enzyme Immunoassay (ELISA).
      Key Applications
      • Dot Blot
      • ELISA
      Application NotesDot Blot Analysis: A representative lot detected Osteocalcin (Glu17) in 3xGlu, Glu17, and 3XGla peptides for human OCN (Courtesy of Mathieu Ferron, Ph.D., Montreal Clinical Research Institute, Montreal, Canada).

      Enzyme Immunoassay Analysis: A representative lot detected Osteocalcin (Glu17) in human serum.
      Biological Information
      ImmunogenA full length recombinant fragment corresponding to 49 amino acids of the mature decarboxylated osteocalcin (with signal peptide and pro regions cleaved off).
      Epitopedomain of:
      Clone5C5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5C5 detects human osteocalcin only when not carboxylated on the first glutamic acid residue (GLU17). It does not react with fully carboxylated osteocalcin.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BGLAP
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight10.96 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceDot Blot Analysis: A 1:250 dilution from a representative lot detected decaboxylated Osteocalcin (Glu17) at a wide range of concentrations (0.8 to 1600 ng).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1978 04054839328763