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MABC1634-100UL Anti-MLKL Antibody, clone 5A6

Anti-MLKL, clone 5A6, Cat. No. MABC1634, is a rat monoclonal antibody that detects MLKL and is tested for use in Immunofluorescence and Western Blotting.

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MABC1634-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1634-100UL
      DescriptionAnti-MLKL Antibody, clone 5A6
      Alternate Names
      • Mixed lineage kinase domain-like protein
      Background InformationMixed lineage kinase domain-like protein (UniProt: Q9D2Y4; also known as MLKL) is encoded by the Mlkl gene (Gene ID: 74568) in murine species. MLKL is a pseudokinase that is a terminal protein in the pro-inflammatory necroptotic cell death program. It plays a critical role in TNFa-induced necroptosis. It is highly expressed in thymus, colon, intestine, liver, spleen, and lung. Its expression is much lower in skeletal muscle, heart and kidney, and it is not detected in the brain. Although it contains a protein kinase domain (aa 192-456), it lacks several residues that are essential for protein kinase activity. It is activated following phosphorylation by RIPK3, which leads to its homotrimerization and localization to the plasma membrane where it binds to highly phosphorylated inositol (InsP6) and disrupts membrane integrity to cause necrotic cell death. Phosphorylation of MLKL at serine 345 is shown to be essential for necroptosis in murine cells. Its interaction with RIPK3 is species specific and mouse MLKL does not interact with human RIPK3. In contrast to human protein, mouse MLKL is not inhibited by necrosulfonamide, because at position 85 it contains a tryptophan residue instead of a cysteine. Under basal conditions, MLKL is shown to reside in small puncta that are distributed evenly throughout the cytoplasm, but it is detected in plasma membrane shortly after its phosphorylation. It contains two coiled coil regions (aa 61-81 and 138-229) and the second coiled coil region is responsible for its oligomerization. Two isoforms of MLKL have been described that are produced by alternative splicing. (Ref.: Samson, AL., et al. (2021). Cell Death Differ. 28(7); 2126-2144; Wang, H., et al (2014). Mol. Cell 54(1); 133-146).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ200
      Applications
      ApplicationAnti-MLKL, clone 5A6, Cat. No. MABC1634, is a rat monoclonal antibody that detects MLKL and is tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected MLKL in Immunofluorescence applications (Samson, A.L., et al. (2021). Cell Death Differ. 28(7):2126-2144).

      Western Blotting Analysis: A representative lot detected MLKL in Western Blotting applications (Samson, A.L., et al. (2021). Cell Death Differ. 28(7):2126-2144).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged full-length recombinant mouse Mixed lineage kinase domain-like protein (MLKL).
      EpitopeC-terminal
      Clone5A6
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 5A6 is a rat monoclonal antibody that detects murine Mixed lineage kinase domain-like protein (MLKL). It targets an epitope within the C-terminal region.
      IsotypeIgG2aλ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Mlkl
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~54 kDa observed; 54.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysates from Mouse dermal fibroblasts.

      Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected MLKL in lysates from wild-type Mouse dermal fibroblasts, but not in lysates from fibroblasts with Mlkl knockout.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1634-100UL 04065268041956