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MABF2044-25UG Anti-MCPyV large T-antigen Antibody, clone CM2B4

Anti-MCPyV large T-antigen, clone CM2B4, Cat. No. MABF2044, is a mouse monoclonal antibody that detects MCPyV large T antigen and has been tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting for the detection of Merkel cell polyomavirus.

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Research Category: Inflammation & Immunology Gene Symbol: N/A UniProt Number: 0
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MABF2044-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2044-25UG
      DescriptionAnti-MCPyV large T-antigen Antibody, clone CM2B4
      Alternate Names
      • Large T antigen
      • MCV Large T antigen
      Background InformationMerkel cells are neuroendocrine cells found in skin that have synaptic contacts with somatosensory afferents. These cells can turn malignant and form an aggressive form of skin cancer, which is known as Merkel cell carcinoma (MCC). A vast majority of MCC are caused by a polyomavirus known as Merkel cell polyomavirus (MCPyV or MCV). The MCPyV genome is reported to undergo clonal integration into the host cell chromosomes of MCC tumors and expresses small T antigen and truncated large T antigen. Full-length MCPyV large T-antigen is a 125 kDa nuclear protein, however, MCPyV T-antigens obtained from MCC have natural truncating mutations, which result in variably-sized, smaller proteins. Based on homology to other polyomaviruses, the MCPyV large and small T antigens are predicted to be oncogenic and contribute directly to the carcinogenesis of MCC. MCPyV large T antigen can serve as a specific marker for MCC.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-MCPyV large T-antigen, clone CM2B4, Cat. No. MABF2044, is a mouse monoclonal antibody that detects MCPyV large T antigen and has been tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting for the detection of Merkel cell polyomavirus.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected MCPyV large T-antigen in Western Blotting applications (Liu, X., et. al. (2011). J Biol Chem. 286(19):17079-90; Rodig, S.J., et. al. (2012) J Clin Invest. 122(12):4645-53). Immunoprecipitation Analysis: A representative lot detected MCPyV large T-antigen in Immunoprecipitation applications (Liu, X., et. al. (2011). J Biol Chem. 286(19):17079-90).
      Immunohistochemistry Analysis: A representative lot detected MCPyV large T-antigen in Immunohistochemistry applications (Rodig, S.J., et. al. (2012) J Clin Invest. 122(12):4645-53; Busam, K.J., et. al. (2009). Am J Surg Pathol . 33(9):1378-85).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to RSRKPSSNASRGA sequence from the Merkel cell polyomavirus large T antigen exon 2 with a C-terminal cystiene.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone CM2B4 is highly specific for MCPyV large T and 57kT isoforms, but does not detect MCPyV small T antigen.
      IsotypeIgG2b
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • N/A
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight45 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MKL-1 cell lysates.
      Western Blotting Analysis: 1 µg/mL of this antibody detected MCPyV large T-antigen in 10 µg of MKL-1 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2044-25UG 04054839476891