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MABN40 Anti-LRRK2 Antibody, clone N138/6

Anti-LRRK2 Antibody, clone N138/6 detects level of LRRK2 & has been published & validated for use in IH & WB.

Anti-LRRK2 Antibody, clone N138/6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling, Neuroscience Research Sub-Category: Kinases & Phosphatases Gene Symbol: LRRK2, PARK8, AURA17, RIPK7, ROCO2 UniProt Number: Q5S007
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MABN40
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN40
      DescriptionAnti-LRRK2 Antibody, clone N138/6
      Alternate Names
      • leucine-rich repeat kinase 2
      • DARDARIN
      • augmented in rheumatoid arthritis 17
      • Parkinson disease (autosomal dominant) 8
      • Dardarin
      Background InformationLeucine-rich repeat kinase 2 (LRRK2) is a 2,527 amino acid protein encoded by the PARK8 gene and belonging to the ROCO family of proteins. This protein family is characterized as having a unique interaction between the Roc domain and the intrinsic GTPase activity along with a COR domain. LRKK2 consists of 5 different domains; a tyrosine kinase-like MAPKKK-related domain, WD40 propeller motif repeats, a Roc (Ras-like small GTPase family) domain, a COR (C-terminal of Roc) domain, and an LRRK. LRRK2 is mainly localized to the cytoplasm and expression within tissue is most highly observed within the brain. It is found in lower levels within the liver, heart, lung, skeletal muscles, placenta, pancreas, and kidney. The exact function of LRRK2 is unknown; however mutations to any of the five domains are the most commonly known causes of Parkinson’s disease, both familial and sporadic. Further study of this protein may lead to a better understanding of the disease’s pathogenesis, as well as potential therapeutic markers.
      References
      Product Information
      FormatPurified
      Control
      • Human placenta and Parkinson's diseased vascular brain tissue
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-LRRK2 Antibody, clone N138/6 detects level of LRRK2 & has been published & validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blot Analysis: A previous lot was used by an independent laboratory in WB. (UC Davis/NIH NeuroMab Facility, www.neuromab.org, neuromab@ucdavis.edu)
      Biological Information
      ImmunogenRecombinant protein corresponding to human LRRK2.
      EpitopeUnknown
      CloneN138/6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes LRRK2.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq].
      Gene Symbol
      • LRRK2
      • PARK8
      • AURA17
      • RIPK7
      • ROCO2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Probable protein kinase whose role is not yet known. May play a role in the phosphorylation of proteins central to Parkinson disease. May also have GTPase activity.

      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

      SUBUNIT STRUCTURE: Interacts with PARK2.

      SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note: Localized in the cytoplasm and associated with cellular membrane structures. Associates with the mitochondrial outer membrane.

      TISSUE SPECIFICTY: Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.

      INVOLVEMENT IN DISEASE: Defects in LRRK2 are the cause of Parkinson disease type 8 (PARK8) [MIM:607060]. A slowly progressive neurodegenerative disorder characterized by bradykinesia, rigidity, resting tremor, postural instability, neuronal loss in the substantia nigra, and the presence of neurofibrillary MAPT (tau)-positive and Lewy bodies in some patients.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

      Contains 16 LRR (leucine-rich) repeats.

      Contains 1 protein kinase domain.

      Contains 1 Roc domain.
      Molecular Weight240 kDa observed. 286 kDa calculated. (Glasson, B., et al. (2006). Ann. Neurol. 59(2):315-322.)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human placenta and Parkinson's diseased vascular brain tissue.

      Immunohistochemistry Analysis: 1:300 dilution of this antibody detected LRRK2 on 10 µg of human placenta and Parkinson's diseased vascular brain tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN40 04053252479045

      Documentation

      Anti-LRRK2 Antibody, clone N138/6 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-LRRK2 Antibody, clone N138/6 Certificates of Analysis

      TitleLot Number
      Anti-LRRK2, clone N138/6 - 2435678 2435678
      Anti-LRRK2, clone N138/6 - 2468163 2468163
      Anti-LRRK2, clone N138/6 - 1959260 1959260
      Anti-LRRK2, clone N138/6 - 1970354 1970354
      Anti-LRRK2, clone N138/6 - 1995603 1995603
      Anti-LRRK2, clone N138/6 - 2090490 2090490
      Anti-LRRK2, clone N138/6 - 2276391 2276391
      Anti-LRRK2, clone N138/6 - 2330630 2330630
      Anti-LRRK2, clone N138/6 - 2491255 2491255
      Anti-LRRK2, clone N138/6 - 3714427 3714427
      Show more

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies