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ABE1447 Anti-Histone H3 Antibody, K36M mutant

Anti-Histone H3, K36M mutant, Cat. No. ABE1447, is a highly specific rabbit polyclonal antibody that targets Histone H3 K36M mutation (H3K36M) and has been tested in Dot Blot, Immunohistochemistry, and Western Blotting.

Anti-Histone H3 Antibody, K36M mutant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ UniProt Number: P84243
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ABE1447
200 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HDB, IHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1447
      DescriptionAnti-Histone H3 Antibody, K36M mutant
      Alternate Names
      • H3K36M
      • Histone H3 K36M mutant
      • H3 histone family
      • member T
      • K36M mutant
      • Histone cluster 3
      • K36M mutant
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci. Somatic missense mutations in histone H3 genes are found in several pediatric brain and bone malignancies, among which ~90% of the chondroblastomas are identified with histone H3 K36M mutation (H3K36M). Loss of H3K36 methylation due to H3K36M mutation causes a genome-wide upregulation of H3K27 methylation, leading to an altered polycomb repressive complex 1 (PRC1) distribution and re-activation of PRC1-suppressed target genes involved in mesenchymal differentiatio
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Histone H3, K36M mutant, Cat. No. ABE1447, is a highly specific rabbit polyclonal antibody that targets Histone H3 K36M mutation (H3K36M) and has been tested in Dot Blot, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Dot Blot
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot immunostained paraffin-embedded sections from human chondroblastoma tissues carrying histone H3 K36M mutation (Courtesy of Denise Bechet and Nada Jabado from McGill University, Montreal, Quebec, Canada).

      Dot Blot Analysis: A representative lot detected histone H3 peptide representing epitope region sequence with K36M mutation (H3K36M), but not peptides representing wild-type sequence with either unmodified methylated K36 residue (Lu, C., et al. (2016). Science. 352(6287):844-849).

      Immunohistochemistry Analysis: A representative lot immunostained paraffin-embedded sections from human soft tissue undifferentiated sarcoma carrying H3.1K36M mutation (Lu, C., et al. (2016). Science. 352(6287):844-849).

      Immunohistochemistry Analysis: A representative lot immunostained paraffin-embedded H3.3K36M mutant tumors from mice xenografts (Lu, C., et al. (2016). Science. 352(6287):844-849).

      Immunohistochemistry Analysis: A representative lot immunostained paraffin-embedded sections from human chondroblastoma tissues carrying histone H3 K36M mutation (Lu, C., et al. (2016). Science. 352(6287):844-849).

      Western Blotting Analysis: A representative lot detected a target band in histone acid extracts from murine mesenchymal progenitor cells expresssing K36M mutant, but not wild-type, histone H3.3 (Lu, C., et al. (2016). Science. 352(6287):844-849).

      Western Blotting Analysis: A representative lot detected a target band in histone acid extracts from human chondroblastoma samples with H3K36M mutation, but not in human chondrocarcinoma samples H3K36M mutation(Lu, C., et al. (2016). Science. 352(6287):844-849).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the target region sequence with K36M mutation.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects histone H3 K36M mutation (H3K36M) without cross-reactivity toward histone H3 without H3K36M mutation.
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse. Predicted to react with a broad-range of species, including Bovine, Porcine, Rabbit, Rat, based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting of H3K36M overexpression in HEK293 transfectants.

      Western Blotting Analysis: A 1:250 dilution of this antibody detected overexpressed K36M mutant, but not wild-type, human histone H3.3 in 10 µg of lysate from HEK293 transfectants.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1447 04054839081477