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MABC1689-25UG Anti-GFP Antibody, clone 2B6

Anti-GFP, clone 2B6, Cat. No. MABC1689, is a mouse monoclonal antibody that detects GFP-conjugated to most proteins and has been tested for use in Immunocytochemistry and Western Blotting.

MABC1689-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1689-25UG
      DescriptionAnti-GFP Antibody, clone 2B6
      Alternate Names
      • Green fluorescent protein
      Background InformationGreen fluorescent protein (UniProt: P42212) is encoded by the GFP gene in Aequorea victoria (Jellyfish). Green fluorescence protein (GFP) is a 27 kDa protein from jellyfish that serves as an energy-transfer acceptor. It transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer and it fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. GFP has an absorption max at 395 nm and the emission peaks at 507 to 510 nm. However, the exact value of emission max depends on the environment of the chromophore. GFP is an important tool in cell biology research that displays stability under fixation conditions and is suitable for a variety of applications, such as tracing cell lineage tracer, reporter of gene expression, or exploring protein-protein interactions.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-GFP, clone 2B6, Cat. No. MABC1689, is a mouse monoclonal antibody that detects GFP-conjugated to most proteins and has been tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GFP in HEK293T cells stabely transfected with GFP-tagged Aquaporin 4.
      Biological Information
      ImmunogenHis-tagged recombinant jellyfish Green fluorescent protein.
      Clone2B6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2B6 is a mouse monoclonal antibody that detects GFP-conjugated to most proteins.
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity NoteAll.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • GFP
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~27 kDa observed; 26.89 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysates from HeLa cells transfected with GFP.

      Western Blotting Analysis: 1 µg/mL of this antibody detected GFP in lysates from HeLa cells transfected with GFP.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1689-25UG 04054839589744

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