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ABN194 Anti-EVI-5 Antibody

This EVI-5 antibody is validated for use in IHC & WB for the detection of the EVI-5 protein.

Anti-EVI-5 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Signaling Neuroscience Gene Symbol: EVI5, NB4S UniProt Number: O60447
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ABN194
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, HIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN194
      DescriptionAnti-EVI-5 Antibody
      Alternate Names
      • Ecotropic viral integration site 5 protein homolog
      • EVI-5
      Background InformationAlso known as Ecotropic viral integration site 5 protein homolog, EVI-5 serves as a key regulator of the cell cycle through time-dependent stabilization of Emi1, FBX05 protein, and APC/C ubiquitination substrates, such as cyclin A. EVI-5 accumulates in early G1 following Plk1 destruction, but is degraded in a Plk1- and ubiquitin-dependent manner in early mitosis. EVI-5 ablation causes premature APC/C activation, SCF(betaTrCP)-dependent Emi1 degradation, and cyclin destruction, all of which leads to cell cycle arrest and cytokinesis failure. It exists as a monomer and a dimer, and it may form complexes with alpha- and gamma-tubulin or interact with the INCENP-aurora B kinase-survivin chromosomal passenger complex. EVI-5 is implicated in abnormal states, such as multiple sclerosis and neuroblastoma.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Mouse and rat brain tissues
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis EVI-5 antibody is validated for use in IHC & WB for the detection of the EVI-5 protein.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blot Analysis: 2 µg/mL from a representative lot detected EVI-5 in NIH/3T3 nuclear extract.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the human EVI-5.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Species Reactivity NoteDemonstrated to react with Mouse and Rat. Predicted to react with Human based upon immunogen design.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EVI5
      • NB4S
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~95 kDa observed. Uncharacterized bands may be observed at ~31 kDa, ~17 kDa, and ~15 kDa in some cell lysates. This protein is known to have three potential phosphorylation sites.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in mouse and rat brain tissues.

      Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected EVI-5 in mouse and rat brain tissues.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN194 04053252831546

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies