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MABE1126 Anti-DNA G-quadruplex (G4) Antibody, clone 1H6

This Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 is validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), ELISA and Flow Cytometry for the detection of DNA G-quadruplex .

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors UniProt Number: N/A
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MABE1126
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AICC, IH(P), ELISA, FCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1126
      DescriptionAnti-DNA G-quadruplex (G4) Antibody, clone 1H6
      Alternate Names
      • DNA G-quadruplex (G4)
      Background InformationSingle-stranded guanine (G)-rich DNA can form stable secondary structures called G-quadruplexes (G-tetrads or G4). Four guanine bases can associate through Hoogsteen hydrogen bonding to form a square planar structure called a guanine tetrad (G-tetrad), and two or more G-tetrads can stack on top of each other to form a G-quadruplex. The quadruplex structure is further stabilized by the presence of a cation, especially potassium, which sits in a central channel between each pair of tetrads. A DNA G-tetrad can be formed within one DNA strand (intramolecular), between two DNA strands (bimolecular), or four DNA strands (tetramolecular). G4 DNA can arise anywhere in the genome where sufficiently long stretches of single-stranded G-rich DNA are exposed during replication, transcription or recombination. Chemical analysis of quadruplex-forming oligonucleotides has revealed the existence of a plethora of dynamic quadruplex structures with varying stabilities. The diverse nature of G4 DNA structures makes it an appealing topic in molecular biomedical research.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 is validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), ELISA and Flow Cytometry for the detection of DNA G-quadruplex .
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • ELISA
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected DNA G-quadruplex (G4) in human pancreas and mouse kidney tissue.

      Immunocytochemistry Analysis: A representative lot detected discrete DNA G-quadruplex (G4) foci on the compact chromosome in the metaphase HeLa and murine embryonic stem cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).

      Immunocytochemistry Analysis: A representative lot detected greatly diminished nuclear DNA G-quadruplex (G4) immunoreactivity upon DNase treatment of paraformaldehyde-fixed and Triton X-100-permeablized HeLa cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).

      Immunocytochemistry Analysis: A representative lot detected G-quadruplex (G4) stabilizing agent telomestatin/TMS treatment-induced nuclear DNA G4 immunoreactivity in chicken DT40 cells lacking the G4-unwinding helicase FANCJ, while the drug enhancing effect was not seen in human FANCJ-expressing DT40 cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).

      Immunocytochemistry Analysis: A representative lot detected a time-dependent increase of nuclear DNA G-quadruplex (G4) foci in U20S cells following the treatment of the DNA G4-stabilizing agent telomestatin/TMS (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).

      ELISA Analysis: Target selectivity of a representative lot was determined by competitive ELISA. Clone 1H6 binds both tetramolecular and unimolecular (intramolecular) DNA G-quadruplex (G4) structures (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).

      Flow Cytometry Analysis: A representative lot detected enhanced DNA G-quadruplex (G4) immunoreactivity in HeLa cells upon treatment with the DNA G4-stabilizing agent TMPyP4, Cat. No. 613560 (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).

      Immunohistochemistry Analysis: A representative lot detected nuclear DNA G-quadruplex (G4) immunoreactivity in various paraffin-embedded human tissue sections, including skin, pancreas, testis, placenta, brain, appendix, colon, and kidney (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
      Biological Information
      ImmunogenKLH-conjugated G-quadruplex DNA derived from oligonucleotides with telomeric repeats.
      Clone1H6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1H6 binds both tetramolecular and unimolecular (intramolecular) DNA G-quadruplex (G4) structures without sequence specificity, while exhibiting significantly lower affinity toward tetramolecular RNA structure or a triplex DNA structure, and little to no affinity toward either non-G4 ssDNA or ddDNA. However, clone 1H6 does not seem to bind the [AGGG(TTAGGG)3] unimolecular G4 structure, indicating a broad selectivity toward many, but not all, DNA G4 structures (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
      IsotypeIgG2bκ
      Species Reactivity
      • All
      Species Reactivity NoteAll Species. Target DNA structure is not species-specific.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: 2.0 µg/mL of this antibody detected DNA G-quadruplex (G4) in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1126 04055977296464

      Documentation

      Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 Certificates of Analysis

      TitleLot Number
      Anti-DNA G-quadruplex (G4), -2728267 2728267
      Anti-DNA G-quadruplex (G4), -2778846 2778846
      Anti-DNA G-quadruplex (G4), -2789412 2789412
      Anti-DNA G-quadruplex (G4), -2816634 2816634
      Anti-DNA G-quadruplex (G4), -Q2613645 Q2613645
      Anti-DNA G-quadruplex (G4), clone 1H6 - 2990486 2990486
      Anti-DNA G-quadruplex (G4), clone 1H6 - 3226258 3226258
      Anti-DNA G-quadruplex (G4), clone 1H6 - 3389940 3389940
      Anti-DNA G-quadruplex (G4), clone 1H6 - 3450598 3450598
      Anti-DNA G-quadruplex (G4), clone 1H6 - 3595117 3595117
      Show more

      References

      Reference overviewPub Med ID
      Detection of G-quadruplex DNA in mammalian cells.
      Henderson, A; Wu, Y; Huang, YC; Chavez, EA; Platt, J; Johnson, FB; Brosh, RM; Sen, D; Lansdorp, PM
      Nucleic acids research  42  860-9  2014

      Show Abstract       		24163102 24163102

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