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ABF1159 Anti-DEFA1

This rabbit polyclonal Anti-DEFA1 Antibody, Cat. No. ABF1159 is validated for use in Flow Cytometry and Western Blotting for the detection of HP 1-56.

Anti-DEFA1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: DEFA1, DEF1, DEFA2, MRS, DEFA1B UniProt Number: P59665
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ABF1159
50 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABF1159
      DescriptionAnti-DEFA1
      Alternate Names
      • HP 1-56
      • Neutrophil defensin HP 1-56 chain
      Background InformationThe human DEFA1 gene (also known as DEF1, DEFA2, MRS, DEFA1B; Gene ID 1667) encodes a 94-amino acid precursor protein (UniProt P59665) with an N-terminal signal peptide (a.a. 1-19) and propeptide (a.a. 20-38) sequence. Posttranslational processing by proteolytic cleavages generates three neutrophil granule peptides, HP 1-56 (a.a. 39-94), neutrophil defensin 1 (HNP-1, HP-1, HP1; a.a. 65-94), and neutrophil defensin 2 (HNP-2, HP-2, HP2; a.a. 66-94). These peptides exhibit several biological activities including the non-oxidative destruction of ingested microorganisms, the inhibition of adrenocorticotropin-stimulated glucocorticoids synthesis, monocyte chemotaxis, the non-cytolytic inhibition of thymidine incorporation in HL-60 promyelocyte-like cells and the stimulation of nifedipine-sensitive calcium channels. The richest source of HP1-56 relative to HP-1 is found in fetal lung tissue. In addition, HP1-56 is found in the HL-60 promyelocytic cell line and in some leukemias, but not in normal mature circulating neutrophils.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rabbit polyclonal Anti-DEFA1 Antibody, Cat. No. ABF1159 is validated for use in Flow Cytometry and Western Blotting for the detection of HP 1-56.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 0.1 µL from a representative lot detected HP 1-56 immunoreactivity in one million 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized HL-60 human promyelocytic leukemia cells.
      Biological Information
      ImmunogenKLH-conjuated linear peptide corresponding to an internal sequence of human HP 1-56.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody targets a region present in prepro-, pro-, and mature HP 1-56 (a.a. 1-94, 20-94, and 39-94, respectively). Target region is absent from neutrophil defensin 1 (HNP-1, HP-1, HP1; a.a. 65-94) and neutrophil defensin 2 (HNP-2, HP-2, HP2; a.a. 66-94).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DEFA1
      • DEF1
      • DEFA2
      • MRS
      • DEFA1B
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~10 kDa observed. 10.20/8.238/6.306 kDa (prepro-/pro-/mature HP 1-56) calculted. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HL-60 cell lysate.

      Western Blotting Analysis: A 1:5000 dilution of this antibody detected HP 1-56 in 10 µg of HL-60 human promyelocytic leukemia cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABF1159 04054839056314