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MABC556 Anti-Cullin-4B/CUL4B Antibody, clone 9B11.1

This Anti-Cullin-4B/CUL4B Antibody, clone 9B11.1 is validated for use in Western Blotting and Immunohistochemistry for the detection of Cullin-4B/CUL4B.

Anti-Cullin-4B/CUL4B Antibody, clone 9B11.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: CUL4B, KIAA0695 UniProt Number: Q13620
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MABC556
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC556
      DescriptionAnti-Cullin-4B/CUL4B Antibody, clone 9B11.1
      Alternate Names
      • Cullin-4B
      • CUL-4B
      Background InformationThe protein CUL4B acts as the scaffold protein within the multi-protein RING E3 ubiquitin protein ligase complexes needed for ubiquitination and subsequent proteasomal degradation of target proteins. CUL4B appears to contribute to the catalysis of ubiquitination by positioning the substrate target protein and the E3 ubiquitin ligase. Among the targets CUL4B is associated with include histone H2A, H3, p53, cyclin E and H4. The cell signaling mTORC1 pathway is also regulated via CUL4b interaction with MLST8 a key player in the mTORC1 pathway. Thus CUL4B is important is proper cell division, and cell cycle function, and is also associated with tumorigenesis in cancer by coordinating with various methylases in promoting specific DNA methylation-based epigenetic silencing in certain cancers. CUL4B is localized in the nucleus as well as the cytoplasm depending upon the context and is widely expressed.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Cullin-4B/CUL4B Antibody, clone 9B11.1 is validated for use in Western Blotting and Immunohistochemistry for the detection of Cullin-4B/CUL4B.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cullin-4B/CUL4B in human kidney tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Cullin-4B/CUL4B.
      Clone9B11.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CUL4B
      • KIAA0695
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~104 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cullin-4B/CUL4B in 10 µg of A549 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC556 04055977112641