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MABT903 Anti-Connexin 43 Antibody, N-terminal Antibody, clone P1E11.B19

This mouse monoclonal Anti-Connexin 43, N-terminal, clone P1E11.B19, Cat. No. MABT903 is tested for use in Immunocytochemistry and Western Blotting for the detection of Connexin-43.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Gja1, Cx43 UniProt Number: P08050
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MABT903
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT903
      DescriptionAnti-Connexin 43 Antibody, N-terminal Antibody, clone P1E11.B19
      Alternate Names
      • Gap junction alpha-1 protein
      • CX43
      • Gap junction 43 kDa heart protein
      Background InformationGap junction alpha-1 protein (UniProt: P08050; also known as Connexin-43, CX43, Gap junction 43 kDa heart protein) is encoded by the Gja1 (also known as Cxn-43) gene (Gene ID: 24392) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 - 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Phosphorylation at Ser325, Ser328 and Ser330 by casein kinase I is reported to modulate gap junction assembly while phosphorylation of Ser368 by protein kinase C gamma isoform leads to disassembly of gap junction plaques and inhibition of gap junction activity. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. In bladder smooth muscle cells, connexin-43 displays a circadian cycle with low levels during the sleep phase.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-Connexin 43, N-terminal, clone P1E11.B19, Cat. No. MABT903 is tested for use in Immunocytochemistry and Western Blotting for the detection of Connexin-43.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Sosinsky, G.E., et. al. (2007). Biochem. 408(3):375-85).

      Western Blotting Analysis (WB): A representative lot detected Connexin 43 in NRK cells (Courtesy of Joell Solan in Paul Lampe s Lab at Fred Hutchinson Cancer Research Center, Seattle, WA).

      Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Kalcheva, N., et. al. (2007). Proc Natl Acad Sci USA. 104(51):20512-6).

      Western Blotting Analysis: 1-4 µg/mL from a representative lot detected Connexin 43 in 10 µg of rat and mouse heart tissue lysates.

      Immunocytochemistry Analysis: A representative lot detected Connexin 43 in Immunocytochemistry applications (Gellhaus, A., et. al. (2004). J Biol Chem. 279(35):36931-42).

      Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Goldberg, G.S., et. al. (2002). J Biol Chem. 277(39):36725-30).

      Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Solan, J.L., et. al. (2003). J Cell Sci. 116(Pt 11):2203-11).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the first 20 amino acids from the N-terminal region of rat connection-43.
      EpitopeN-terminus
      CloneP1E11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone P1E11.B19 detects connexin-43 in most mammalian species. It targets an epitope with in the first 20 amino acids from the N-terminal end.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Bovine, Canine, Chicken, Hamster, Horse, Porcine, Rabbit, and Rhesus Macaque based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Gja1
      • Cx43
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~43 kDa observed; 43.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human heart tissue lysates.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Connexin 43 in 10 µg of human heart tissue lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT903 04054839199363

      Documentation

      Anti-Connexin 43 Antibody, N-terminal Antibody, clone P1E11.B19 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Connexin 43 Antibody, N-terminal Antibody, clone P1E11.B19 Certificates of Analysis

      TitleLot Number
      Anti-Connexin 43, N-terminal, clone P1E11.B19 3083524
      Anti-Connexin 43, N-terminal, clone P1E11.B19 - 3198419 3198419
      Anti-Connexin 43, N-terminal, clone P1E11.B19 - 3666810 3666810
      Anti-Connexin 43, N-terminal, clone P1E11.B19 - 3804845 3804845
      Anti-Connexin 43, N-terminal, clone P1E11.B19 - 3883668 3883668
      Anti-Connexin 43, N-terminal, clone P1E11.B19 - 3888842 3888842
      Anti-Connexin 43, N-terminal, clone P1E11.B19 - 3980287 3980287
      Anti-Connexin 43, N-terminal, clone P1E11.B19 - 4107729 4107729
      Anti-Connexin 43, N-terminal, clone P1E11.B19 Monoclonal Antibody Q2841560