Millipore Sigma Vibrant Logo

AB1727 Anti-Connexin 43 Antibody

View Products on Sigmaaldrich.com
AB1727
50 µg  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Fulfillment and delivery delayed
Fulfillment and delivery delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, M, RELISA, IP, WB, ICC, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1727
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Connexin 43 Antibody
      OverviewMouse Connexin 43 is a 382 amino acid gap junction protein withmouse connexin

      a predicted M.W. of ~43 kDa. It is prominently expressed in heart (see reviews: Kumar & Giula 1996; White et al. 1995; Evans 1994; Beyer et al. 1990).
      Alternate Names
      • Gap Junction alpha-1 Protein (CxA-1)
      Product Information
      FormatAffinity Purified
      PresentationAffinity-purified using peptide-Sepharose column chromatography and supplied in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Connexin 43 Antibody is validated for use in ELISA, IP, WB, IC for the detection of Connexin 43.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesELISA: 1:10,000-100,000 using 50 - 100 ng Cx43 control peptide per well.
      Immunocytochemistry: not tested. It is recommended that the antibody be tried at 2-20μg/mL in formaldehyde fixed (Beyer et al. 1985; Nicholson et al. 1985; John et al. 1991; Fishman et al. 1990).
      Immunoblotting: 1-10μg/mL using Chemiluminescence technique.
      Optimal working dilutions must be determined by end user.
      Immunohistochemistry: A 1:50 dilution of this antibody detected Connexin 43 in sections from mouse heart tissue pretreated with Tris-EDTA buffer, pH 9.0. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide
      corresponding to amino acids 360-382 within
      the C-terminus of mouse connexin 43.
      HostRabbit
      SpecificityMouse Cx43 immunogenic peptide sequence is specific for Cx43 and no significant homology is seen with other connexins. The mouse Cx43 peptide sequence shows 100% conserved with rat and bovine, and 84% with chicken and human (16/19 aa) Cx43 (Beyer et al. 1985; Nicholson et al. 1985; John et al. 1991; Fishman et al. 1990).
      Species Reactivity
      • Bovine
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations.
      Gene Symbol
      • GJA1
      • GJAL
      • Cx43
      • DFNB38
      • SDTY3
      • CX43
      • ODD
      • ODDD
      • ODOD
      • Connexin-43
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P17302 # One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
      SIZE: 382 amino acids; 43008 Da
      SUBUNIT: A connexon is composed of a hexamer of connexins. Interacts with SGSM3.
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
      TISSUE SPECIFICITY: Expressed in the heart and fetal cochlea.
      DISEASE: SwissProt: P17302 # Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type III and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances. & Defects in GJA1 may be the cause of syndactyly type III (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected. & Defects in GJA1 a the cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
      SIMILARITY: SwissProt: P17302 ## Belongs to the connexin family. Alpha-type (group II) subfamily.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size50 µg
      Global Trade Item Number
      Catalogue Number GTIN
      AB1727 04053252671654