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MAB5284 Anti-Chondroitin Sulfate Proteoglycan Antibody, Brain (core protein), clone Cat-301

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MAB5284
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Gp, H, Ht, R, Fe, Gr, PmIP, WB, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB5284
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Chondroitin Sulfate Proteoglycan Antibody, Brain (core protein), clone Cat-301
      Alternate Names
      • CSPG
      Background InformationThe antigen recognized by this clone, CAT-301 is a proteoglycan that is developmentaly regulated, high molecular chondroitin sulphate proteoglycan found on the extracellular surface of mammalian neurons. It is expressed late in development and although no definitive role has been identified for CAT-301, it is believed to have a role in the stabilisation of synaptic structure.

      The name derives from the name of the monoclonal antibody originally used to identified by its expression, which was detected immunocytochemicaly



      Disruption of the normal patterns of neuronal activity during the critical early postnatal period by physical or biochemical means results in a large and irreversible reduction in levels of CAT-301. Similar intervention in mature animals has no effect



      CAT-301 is related to aggrecan from articular cartilage. The CAT-301 antibody immunoreacts with aggrecan, and the chondroitinase treated aggrecan and CAT-301 both have apparent sizes of 550kDa. However CAT- 301 is not glycosylated by keratan sulphate, as aggrecan is.
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid. Buffer = 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Chondroitin Sulfate Proteoglycan Antibody, Brain (core protein), clone Cat-301 detects level of Chondroitin Sulfate Proteoglycan & has been published & validated for use in IP, WB, IC, IH.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:500-1:2,000 on 4% paraformaldehyde fixed frozen tissue.

      Immunocytochemistry

      Immunoblot

      Immunoprecipitation

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenFeline spinal cord fixed gray matter.
      EpitopeBrain (core protein)
      CloneCat-301
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityChondroitin sulfate proteoglycan (CSPG), brain core protein.
      IsotypeIgG1
      Species Reactivity
      • Guinea Pig
      • Human
      • Hamster
      • Rat
      • Feline
      • Gerbil
      • Primate
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.
      Gene Symbol
      • ACAN
      • MSK16
      • SEDK
      • CSPCP
      • AGCAN
      • AGC1
      • CSPG1
      • CSPGCP
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16112 # This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
      SIZE: 2415 amino acids; 250193 Da
      SUBUNIT: Interacts with FBLN1 (By similarity).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
      TISSUE SPECIFICITY: Restricted to cartilages.
      DEVELOPMENTAL STAGE: Expression was detected in chondrocytes throughout the developing skeleton.
      DOMAIN: SwissProt: P16112 Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.
      PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.
      DISEASE: SwissProt: P16112 # Defects in AGC1 are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK) [MIM:608361]. Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy.
      SIMILARITY: Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 1 EGF-like domain. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 4 Link domains. & Contains 1 Sushi (CCP/SCR) domain.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB5284 04053252666223