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MABS1727 Anti-CYB5R3 Antibody, clone 1D9.2

Detect CYB5R3 using this mouse monoclonal Anti-CYB5R3 Antibody, clone 1D9.2, Cat. No. MABS1727, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-CYB5R3 Antibody, clone 1D9.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: CYB5R3, DIA1 UniProt Number: P00387
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MABS1727
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1727
      DescriptionAnti-CYB5R3 Antibody, clone 1D9.2
      Alternate Names
      • NADH-cytochrome b5 reductase 3
      • B5R
      • Cytochrome b5 reductase
      • Diaphorase-1
      Background InformationNADH-cytochrome b5 reductase 3 (EC 1.6.2.2; UniProt P00387; also known as B5R, Cytochrome b5 reductase, Diaphorase-1) is encoded by the CYB5R3 (also known as DIA1) gene (Gene ID 1727) in human. Cytochrome b5 reductase is a flavoprotein that serves as electron donor for cytochrome b5, a ubiquitous electron carrier and participates in a variety of metabolic pathways, including desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. It is produced as two different isoforms that have different localizations. The membrane-bound form is found in somatic cells where it is anchored to the endoplasmic reticulum and mitochondrial membranes. The soluble form is found in erythrocytes. Mutations in the CYB5R3 gene result in deficiency in NADH-cytochrome b5 reductase+C20 in erythrocytes, which causes methemoglobinemia types I and II that are characterized by the presence of excessive amounts of methemoglobin in blood cells and their reduced capacity to carry oxygen. Ref.: Elahian, F., et al. (2014). Crit. Rev Biotechnol. 34, 134-143.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect CYB5R3 using this mouse monoclonal Anti-CYB5R3 Antibody, clone 1D9.2, Cat. No. MABS1727, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected CYB5R3 in human kidney, skeletal muscle, and tonsil tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human CYB5R3 C-terminal fragment.
      Clone1D9.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityTarget epitope is present in both the ER/mitochondria membrane-bound and cytosolic forms of human CYB5R3.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CYB5R3
      • DIA1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~34 kDa observed. 34.10 kDa (isoform 1 or M; Membrane-bound form with Met1 removed, a.a. 2-301), 31.26 kDa (Cytosolic form, a.a. 27-301), 31.63 kDa (isoform 2 or S), 38.23 kDa (isoform 3) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 celll lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected CYB5R3 in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1727 04054839105548