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MABE1915-25UG Anti-CPEB4 Antibody, clone A4

Anti-CPEB4, clone A4, Cat. No. MABE1915, is a mouse monoclonal antibody that detects CPEB4 and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

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MABE1915-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1915-25UG
      DescriptionAnti-CPEB4 Antibody, clone A4
      Alternate Names
      • Cytoplasmic polyadenylation element binding protein 4
      Background InformationCytoplasmic polyadenylation element binding protein 4 (UniProt: D3ZKL3; also known as CPEB4) is encoded by the Cpeb4 (also known as rCG_33472) gene (Gene ID: 303010) in rat. The CPEB family of RNA-binding proteins regulate translation in various tissues in vertebrates. They shuttle between nucleocytoplasmic compartments with longer retention in the cytoplasm where they regulate target RNA translation. CPEB4 is an RNA-binding protein that promotes polyadenylation and translation of target RNAs and contributes to meiotic and mitotic progression. It acts as a survival factor exclusively for early postnatal granule cells in the olfactory bulb. CPEB4 is reported to interact with adaptor protein vinexin by binding to its first two SH3 domains and this interaction leads to vinexin localization in stress granules. It is shown that CPEB4 recruits vinexin to stress granules in response to adverse conditions, such as heat shock or arsenite-induced stress. CPEB4 is also shown to be essential for cell survival and becomes nuclear in response to focal ischemia in vivo and when cultured neurons are deprived of oxygen and glucose. (Ref.: Tseng, CS., et al. (2017). Cell Reports 21, 2264-2276; Chang, YW., and Huang, YS. (2014). PLoS ONE 9(9); e107961; Kan, MC., et al. (2010). Mol. Cell Biol. 30(24); 5658-5671).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CPEB4, clone A4, Cat. No. MABE1915, is a mouse monoclonal antibody that detects CPEB4 and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesTested Applications

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CPEB4 in A375 and HepG2 cell lysates.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated CPEB4 in Immunoprecipitation applications (Chang, Y.W., et al. (2014). PLoS One. 9(9):e107961).

      Immunocytochemistry Analysis: A representative lot detected CPEB4 in Immunocytochemistry applications (Chang, Y.W., et al. (2014). PLoS One. 9(9):e107961).

      Western Blotting Analysis: A representative lot detected CPEB4 in Western Blotting applications (Chang, Y.W., et al. (2014). PLoS One. 9(9):e107961; Tseng, C-S., et al. (2017). Cell Rep. 21(8):2264-2276).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant fragment corresponding to the first 458 amino acids from the N-terminal region of rat CPEB4.
      EpitopeN-terminal
      CloneA4
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone A4 is a mouse monoclonal antibody that detects Cytoplasmic polyadenylation element binding protein 4 (CPEB4). It targets an epitope within the N-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cpeb4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~79 kDa observed; 79.15 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Neuro-2A cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CPEB4 in Neuro-2A cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1915-25UG 04065267404097