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ABE1857 Anti-CEP135

Anti-CEP135, Cat. No. ABE1857, is a highly specific rabbit polyclonal antibody that targets Centrosomal protein of 135 kDa and has been tested in Electron Microscopy, Immunocytochemistry, and Western Blotting.

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ABE1857
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE1857
      DescriptionAnti-CEP135
      Alternate Names
      • Centrosomal protoein of 135 kDa
      • Centrosomal protein 4
      Background InformationCentrosomal protein of 135 kDa (UniProt: Q66GS9; also known as Cep135, Centrosomal protein 4) is encoded by the CEP135 (also known as CEP4, KIAA0635) gene (Gene ID: 9662) in human. Cep135 is a centrosomal protein that is involved in centriole biogenesis. Human Cep135 is reported to contain two-stranded coiled-coil domains that are critical for microtubule binding. Cep135 acts as a scaffolding protein during early centriole biogenesis and is required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. It also plays a role in centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. Mutations in CEP135 gene are associated with autosomal recessive primary microcephaly-8, a disease characterized by intellectual disability, markedly reduced brain weight, and small cerebral cortex.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CEP135, Cat. No. ABE1857, is a highly specific rabbit polyclonal antibody that targets Centrosomal protein of 135 kDa and has been tested in Electron Microscopy, Immunocytochemistry, and Western Blotting.
      Key Applications
      • Electron Microscopy
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CEP135 in synchronized U2OS cells.

      Electron Microscopy Analysis: A representative lot detected CEP135 in Electron Microscopy applications (Kleylein-Sohn, J., et. al. (2007). Dev Cell. 13(2):190-202).

      Immunocytochemistry Analysis: A representative lot detected CEP135 in Immunocytochemistry applications (Kleylein-Sohn, J., et. al. (2007). Dev Cell. 13(2):190-202; Guderian, G., et. al. (2010). J Cell Sci. 123(Pt 13):2163-9; Sonnen, K.F., et. al. (2012). Biol Open. 1(10):965-76).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 492 amino acids from the C-termnal region of human Cep135.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects centrosomal protein of 135 kDa in human cells. It targets an epitope within 492 amino acids from the C-terminal half.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CEP135
      • CEP4
      • KIAA0635
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight~150 kDa observed; 133.49 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U2OS cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected CEP135 in 10 µg of U2OS cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1857 04054839233180

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