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217595 Anti-CD40L Mouse mAb (24-31)

Anti-CD40L, mouse monoclonal, clone 24-32, recognizes the ~36 kDa CD40L (gp39) on activated CD3+ human PBL. It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies.

Anti-CD40L Mouse mAb (24-31) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-gp39

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217595
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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217595-100UG
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      Description
      OverviewRecognizes the ~36 kDa CD40L (gp39) protein on activated CD3+ human PBL.
      Note: 1 T = 1 test.
      Catalogue Number217595
      Brand Family Calbiochem®
      SynonymsAnti-gp39
      References
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Preservative0.5 mg/ml gentamycin sulfate
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Oledzka, G., et al. 2007. Mol. Immunol. 44, 741.
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Neutralization Studies
      Application NotesFlow Cytometry (10 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      Neutralization Studies (see comments)
      Immunoblotting (see application references)
      Application CommentsThis antibody immunoprecipitates the ~39 kDa CD40L (gp39). It will stain activated CD3+ human PBL and will functionally block MLR, spg39 induced B-cell proliferation and T-cell dependent B-cell differentiation. FACS was performed on 5x105 ficoll-prepared PHA/PMA stimulated human peripheral white blood cells using anti-CD40L at 10 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody has also been reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenhuman sgp39 fusion protein
      ImmunogenHuman
      Clone24-31
      HostMouse
      Purity≥95% by SDS-PAGE
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      217595-100UG 04055977202885

      Documentation

      Anti-CD40L Mouse mAb (24-31) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD40L Mouse mAb (24-31) Certificates of Analysis

      TitleLot Number
      217595
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      SynonymsAnti-gp39
      ApplicationFlow Cytometry (10 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      Neutralization Studies (see comments)
      Immunoblotting (see application references)
      DescriptionProtein A affinity purified mouse monoclonal antibody. Recognizes the ~39-40 kDa CD40L protein.
      BackgroundHuman CD40 ligand (CD40L) is a member of the tumor necrosis factor (TNF) family expressed on the surface of activated T-cells.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman sgp39 fusion protein
      Clone24-31
      IsotypeIgG₁
      Specieshuman
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥95% by SDS-PAGE
      Preservative0.5 mg/ml gentamycin sulfate
      CommentsThis antibody immunoprecipitates the ~39 kDa CD40L (gp39). It will stain activated CD3+ human PBL and will functionally block MLR, spg39 induced B-cell proliferation and T-cell dependent B-cell differentiation. FACS was performed on 5x105 ficoll-prepared PHA/PMA stimulated human peripheral white blood cells using anti-CD40L at 10 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody has also been reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      Application referencesImmunoblotting Oledzka, G., et al. 2007. Mol. Immunol. 44, 741.