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217640 Anti-CD11a Mouse mAb (38)

Anti-CD11a, mouse monoclonal, clone 38, recognizes the ~180 kDa I domain of the αL integrin subunit in Molt-4 cells. It is validated for FC, IP, IHC on frozen sections and for cell inhibition assays.

Anti-CD11a Mouse mAb (38) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-αL Integrin, Anti-LFA-1

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217640
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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217640-100UG
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      Description
      OverviewRecognizes the ~180 kDa I domain of the αL integrin subunit in Molt-4 human tumor cells.
      Catalogue Number217640
      Brand Family Calbiochem®
      SynonymsAnti-αL Integrin, Anti-LFA-1
      References
      ReferencesHolness, C.L., et al. 1995. J. Biol. Chem. 270, 877.
      Landis, R.C., et al. 1994. J. Cell. Biol. 126, 529.
      Dransfield, I., and Hogg, N. 1989. EMBO J. 8, 3759.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Negative controlNalm-6 cells
      Positive controlMolt-4 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Cell Inhibition Assays
      Flow Cytometry
      Frozen Sections
      Immunoprecipitation
      Application NotesCell Inhibition Assays (1-5 µg/ml, see comments)
      Flow Cytometry (5 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      Application CommentsAlso blocks binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured Molt-4 human tumor cells using anti-CD11a antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenhuman monocytes
      ImmunogenHuman
      Clone38
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      217640-100UG 04055977202922

      Documentation

      Anti-CD11a Mouse mAb (38) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD11a Mouse mAb (38) Certificates of Analysis

      TitleLot Number
      217640

      References

      Reference overview
      Holness, C.L., et al. 1995. J. Biol. Chem. 270, 877.
      Landis, R.C., et al. 1994. J. Cell. Biol. 126, 529.
      Dransfield, I., and Hogg, N. 1989. EMBO J. 8, 3759.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      SynonymsAnti-αL Integrin, Anti-LFA-1
      ApplicationCell Inhibition Assays (1-5 µg/ml, see comments)
      Flow Cytometry (5 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      DescriptionProtein A purified mouse monoclonal antibody. Recognizes the I domain of the ~180 kDa integrin α L subunit (CD11a).
      BackgroundCD11a (αL integrin) complexes with CD18 (β2 integrin) to form the LFA-1 heterodimer adhesion molecule which binds to three ICAM's 1,2, and 3, also designated CD54, CD102, and CD50, respectively. LFA-1 is expressed on lymphocytes, monocytes, and neutrophils and plays a role in cell-cell adhesion.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman monocytes
      Clone38
      IsotypeIgG2a
      Specieshuman
      Positive controlMolt-4 cells
      Negative controlNalm-6 cells
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAlso blocks binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured Molt-4 human tumor cells using anti-CD11a antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling
      ReferencesHolness, C.L., et al. 1995. J. Biol. Chem. 270, 877.
      Landis, R.C., et al. 1994. J. Cell. Biol. 126, 529.
      Dransfield, I., and Hogg, N. 1989. EMBO J. 8, 3759.