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ABS1023 Anti-Angiomotin Antibody

Anti-Angiomotin Antibody | ABS1023 is an antibody against Angiomotin Antibody for use in Western Blotting, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP).

Anti-Angiomotin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: AMOT UniProt Number: Q4VCS5
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ABS1023
100 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, IF, ICC, ChIPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1023
      DescriptionAnti-Angiomotin Antibody
      Alternate Names
      • Angiomotin
      Background InformationAngiomotin is a cell surface protein that plays a central role in tight junction maintenance and the uptake of proteins at tight junctions but also appears to regulate endothelial cell response and their migration toward growth factors as well as their response to angiostatin. Thus Angiomotin plays a role in blood vessel formation, cell migration, protein localization, chemotaxis, cellular polarity and many more cell-movement/migration activities. Angiomotin is a component of a multi-protein complex associated at tight junctions that includes ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3 proteins. Angiomotin is expressed in endothelial cells and found in placenta, blood vessels and skeletal muscle tissue, as well as at the leading edge of lamellipodia and cellular processes.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Angiomotin Antibody | ABS1023 is an antibody against Angiomotin Antibody for use in Western Blotting, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP).
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunofluorescence
      • Immunocytochemistry
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blotting Analysis: A representative lot detected phospho Angiomotin (Ser175) in HEK293 transfected with AMOT cell lysate (Dr. Joseph Kissil, Scripps Research Institute, Florida).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated Angiomotin in HEK293 cells expressing equal amount of HA-Merlin and Myc-Rich1, untreated (−) or Doxycyline induced (+) RT4-67 cells (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540).
      Western Blotting Analysis: A representative lot detected Angiomotin in Flag-IP and total cell lysate from HEK293 cells transfected with Flag-tagged wild-type (WT) or patient-derived mutant Merlin alleles, HEK293, ERK activation in Nf2−/− SC4 cells or Merlin-reconstituted SC4 cells (Nf2−/−+retroNf2) (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540).
      Western Blotting Analysis: A representative lot detected Angiomotin in HEK293, MEFs treated with Adeno (−) or
      Adeno-Cre (+) virus (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).
      Immunofluorescence Analysis: A representative lot detected Angiomotin in epithelial cells (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540).
      Immunofluorescence Analysis: A representative lot detected Angiomotin in liver from Amot KO mice (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).
      Immunocytochemistry Analysis: A representative lot detected Angiomotin in SC4 cells (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).
      Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Angiomotin in HEK293 cells (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).
      Biological Information
      ImmunogenLinear peptide corresponding to human Angiomotin.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis antibody detects p80 and p130 forms of AMOT
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AMOT
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight150/80 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 transfected with LATS cell lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Angiomotin in 10 µg of HEK293 transfected with LATS cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1023 04055977126938

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies