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MABS2049-25UG Anti-Adenylate Cyclase 5 Antibody, clone 19D5.C1

Anti-Adenylate Cyclase 5, clone 19D5.C1, Cat. No. MABS2049, is a mouse monoclonal antibody that detects adenylate cyclase 5 and has been tested for use in ELISA and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Adcy5 UniProt Number: P84309
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MABS2049-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, Porcine, RELISA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS2049-25UG
      DescriptionAnti-Adenylate Cyclase 5 Antibody, clone 19D5.C1
      Alternate Names
      • Adenylate cyclase type 5
      • EC: 4.6.1.1
      • ATP pyrophosphate-lyase 5
      • Adenylate cyclase type V
      • Adenylyl cyclase 5
      Background InformationAdenylate cyclase type 5 (P84309; also known as EC: 4.6.1.1, ATP pyrophosphate-lyase 5, Adenylate cyclase type V, Adenylyl cyclase 5, AC5) is encoded by the Adcy5 gene (Gene ID: 224129) in murine species. Adenylate cyclase catalyzes the formation of cyclic AMP in response to G-protein signaling. It regulates the increase of free cytosolic calcium in response to increased blood glucose levels and contributes to the regulation of calcium-dependent insulin secretion. Adenylyl cyclases are typically made up of two clusters of six transmembrane domains separating two intracellular, overlapping catalytic domains. The amino acid sequence of each cytoplasmic domain that contains the nucleotide (ATP)-binding site, is well conserved among various subtypes. Thus far 9 different hormone-sensitive, membrane-bound AC isoforms have been identified. Adenylyl Cyclase 5 deficiency is shown to protect against diet-induced obesity and insulin resistance in mice and extends their life span. Adenylate cyclase 5 knockout mice display signs of reduced stress and protection against the stress of chronic pressure overload on heart. Adenylate cyclase 5 activity is stimulated by forskolin and is inhibited by adenosine and ATP analogs. (Ref.: Ho, D., et al. (2015). Diabetes. 64(7); 2636-2645. Vatner, SF., et al. (2015). Oxid. Med. Cell. Longev. 2015:250310).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Adenylate Cyclase 5, clone 19D5.C1, Cat. No. MABS2049, is a mouse monoclonal antibody that detects adenylate cyclase 5 and has been tested for use in ELISA and Western Blotting.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Adenylyl Cyclase 5 in Western Blotting applications (Hu, C.L., et. al. (2009). Am J Physiol Heart Circ Physiol. 297(5):H1776-82; De Lorenzo, M.S., et. al. (2014). Aging Cell. 13(1):102-10).

      ELISA Analysis: A representative lot detected Adenylyl Cyclase 5 in ELISA applications (Hu, C.L., et. al. (2009). Am J Physiol Heart Circ Physiol. 297(5):H1776-82).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 20 amino acids from the cytoplasmic domain of murine adenylate cyclase V.
      Epitopecytoplasmic domain
      Clone19D5.C1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 19D5.C1 detects Adnylate Cyclase 5 in murine, rat, and procine cells. It targets an epitope within 20 amino acids from the cytoplasmic domain.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Porcine
      • Rat
      Species Reactivity NoteMouse, Rat, Porcine. Predicted to react with Human based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Adcy5
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~150 kDa observed; 139.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain membrane preparations.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Adenylate Cyclase 5 in mouse brain membrane preparations.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2049-25UG 04054839469022