Millipore Sigma Vibrant Logo

07-1286 Anti-ATM Antibody

View Products on Sigmaaldrich.com
07-1286
200 µL  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Fulfillment and delivery delayed
Fulfillment and delivery delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1286
      Trade Name
      • Upstate
      DescriptionAnti-ATM Antibody
      Alternate Names
      • A-T, mutated
      • AT mutated
      • ataxia telangiectasia mutated
      • ataxia telangiectasia mutated (includes complementation groups A, C and D)
      • ataxia telangiectasia mutated protein
      • ATM
      • human phosphatidylinositol 3-kinase homolog
      • serine-protein kinase ATM
      • TEL1
      • telomere maintenance 1, homolog
      Background InformationATM (Ataxia Telangiectasia Mutated kinase) and ATR (Ataxia Telangiectasia and Rad3-related kinase) are related kinases that regulate cell cycle checkpoints and DNA repair. ATM is activated in response to DNA damage and serves to arrest further cell division before the damage can be repaired. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM include p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. ATM activates p53, increasing p21/Cip1/Waf1 levels, thus blocking activation of Cdk2. That results in Rb hypophosphorylation and blockage of the G1/S transition. Separately, ATM also phosphorylates and activates Chk1, which phosphorylates Cdc25C. This inactivates Cdc25C and prevents it from dephosphorylating the inhibitory phosphotyrosine residue on cdc2/Cdk1, thus preventing the G2/M transition. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.
      Product Information
      FormatAffinity Purified
      Control
      • HeLa nuclear extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-ATM Antibody detects level of ATM & has been published & validated for use in WB & IF.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A 1:5,000 dilution from a representative lot detected ATM in 10 µg of HeLa nuclear extract.
      Immunocytochemistry Analysis: A representative lot detected ATM in HeLa and NIH/3T3 cells.
      Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected ATM in NIH/3T3 cells.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to sequence derived from the Abl-interacting domain of human ATM.
      EpitopeAbl-interacting domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityDetects ATM.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Bovine, Rat, Horse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1.
      This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • AT1
      • ATA
      • ATC
      • ATD
      • ATDC
      • ATE
      • DKFZp781A0353 2
      • EC 2.7.11.1 3
      • MGC74674
      • TEL1
      • TELO1
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight>260 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected ATM in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size200 µL
      Global Trade Item Number
      Catalogue Number GTIN
      07-1286 04053252588624