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ABE387 Anti-ATF4 Antibody

Anti-ATF4 is an antibody targeting the ATF4 protein, validated for use in WB & ChIP.

Anti-ATF4 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: ATF4, CREB2, TXREB UniProt Number: P18848
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ABE387
100 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ChIPRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABE387
      DescriptionAnti-ATF4 Antibody
      Alternate Names
      • Cyclic AMP-dependent transcription factor ATF-4
      • cAMP-dependent transcription factor ATF-4
      • Activating transcription factor 4
      • Cyclic AMP-responsive element-binding protein 2
      • CREB-2
      • cAMP-responsive element-binding protein 2
        DNA-binding protein TAXREB67
      • Tax-responsive enhancer element-binding protein 67
      • TaxREB67
      Background InformationCyclic AMP-dependent transcription factor ATF-4 (ATF4) is a basic leucine-zipper (bZip) transcription factor, which regulates amino acid metabolism, DNA damage repair, chromatin remodeling, and apoptosis in response to cellular and ER stress. ATF4 works with various proteins, such as C/EBP homology protein (CHOP), aspargine synthetase (ASNS), and cAMP response element (CRE) among others to mediate cellular stress. ATF4 also regulates glucose homeostasis by suppressing beta-cell proliferation and insulin production. Furthermore, ATF4 targets the histone demethylase JMJD3 to alter chromatin structure and enhance gene transcription in response to amino acid deprivation.
      References
      Product Information
      FormatSerum
      Control
      • HisOH treated HepG2 cell lysate
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-ATF4 is an antibody targeting the ATF4 protein, validated for use in WB & ChIP.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected ATF4 in HEPG2 cell lysates (Su, N. and Kilberg, M. S., et al. (2008). J Biol Chem. 283(50):351016-35117.; Fu, L., et al. (2011). J Biol Chem. 286(42):36724-36738.).

      Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected ATF4 in HEPG2 and MEF cell lysates (Shan, J., et al. (2012). J Biol Chem. 287(43):36393-36403.).
      Biological Information
      ImmunogenRecombinant protein corresponding to human ATF4.
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ATF4
      • CREB2
      • TXREB
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~48 kDa observed. The calculated molecular weight is 39 kDa; however, this protein may be observed at ~48 kDa in human tissue lysates and ~45 kDa in mouse cell lysates due to post-translational modification. Uncharacterized bands may be observed at ~57 kDa, ~59 kDa, ~75 kDa, and ~100 kDa in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and HisOH treated HepG2 cell lysate.

      Western Blot Analysis: A 1:2,000 dilution from a representative lot detected ATF4 in 10 µg of HisOH treated HepG2 cell lysate (HisOH was used to induce cellular stress mediated eIF2a phosphorylation, allowing for subsequent detection of ATF4. For enhanced performance, end-users may try higher dilutions of this antibody. For better results, 1% SDS was used during the secondary antibody incubation step.)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE387 04053252850769

      Documentation

      Anti-ATF4 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-ATF4 Antibody Certificates of Analysis

      TitleLot Number
      Anti-ATF4 3078493
      Anti-ATF4 - 3398042 3398042
      Anti-ATF4 - 3495620 3495620
      Anti-ATF4 - 3707450 3707450
      Anti-ATF4 - 4070112 4070112
      Anti-ATF4 - Q2116528 Q2116528
      Anti-ATF4 -2501504 2501504
      Anti-ATF4 -2736396 2736396
      Anti-ATF4 Polyclonal Antibody 3120174
      Anti-ATF4 Polyclonal Antibody 3037998

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics