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ABC1435-25UL Anti-ALIX

Anti-ALIX, Cat. No. ABC1435, is a rabbit polyclonal antibody that detects Programmed cell death 6-interacting protein and has been tested ted for use in Immunocytochemistry and Western Blotting.

Research Category: Apoptosis & Cancer Gene Symbol: PDCD6IP, AIP1, ALIX, KIAA1375 UniProt Number: Q8WUM4
ABC1435-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC1435-25UL
      DescriptionAnti-ALIX
      Alternate Names
      • Programmed cell death 6-interacting protein
      • PDCD6-interacting protein
      • ALG-2-interacting protein 1
      • ALG-2-interacting protein X
      • Hp95
      Background InformationProgrammed cell death 6-interacting protein (UniProt: Q8WUM4; also known as PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95) is encoded by the PDCD6IP (also known as AIP1, ALIX, KIAA1375) gene (gene ID: 10015) in human. ALIX, the mammalian orthologue of xenopus Xp95 and yeast BRO1, is an abundant adaptor protein involved in diverse cellular processes, including actin-based cytoskeleton assembly, integrin-mediated cell adhesion, and ESCRT- (endosomal sorting complex required for transport-) dependent membrane trafficking. ALIX is a Class E VPS protein involved in concentration and sorting of cargo proteins of the multi-vesicular body (MVB) for incorporation into intralumenal vesicles that are generated by invagination and scission from the limiting membrane of the endosome. Sorting of endocytosed membrane proteins by MVBs requires coordinated cargo recognition and assembly of membrane-sculpturing machinery that buds cargo-loaded membrane vesicles into the lumen of endosomes. MVB-mediated sorting is driven by five distinct ESCRT complexes (ESCRT-0, -I, -II and -III, and the Vps4 complex) and associated proteins. ALIX and charged MVB protein 4 (CHMP4), the mammalian orthologue of yeast Snf7, are binding partners involved in a variety of ESCRT-III-mediated membrane-remodeling processes in mammalian cells, including retroviral budding, cytokinetic abscission, biogenesis of exosomes, plasma membrane repair, and ubiquitin-independent MVB sorting of protease-activated receptor 1 (PAR1). In the absence of binding partners, an intramolecular interaction between the Patch 2 region in the Bro1 domain (aa 3-392) and the TSG101-docking site (aa 717-720) in the Pro-rich domain (aa 717-860) locks ALIX in a conformation inaccessible for interaction with CHMP4 and retroviral Gag proteins.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-ALIX, Cat. No. ABC1435, is a rabbit polyclonal antibody that detects Programmed cell death 6-interacting protein and has been tested ted for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected ALIX in 10 µg of  HEK293 cell lysate.
      Immunocytochemistry Analysis: A representative lot detected ALIX in Immunocytochemistry applications (Morita, E., et. al. (2007). EMBO J. 26(19):4215-27).
      Western Blotting Analysis: A representative lot detected ALIX in Western Blotting applications (Morita, E., et. al. (2007). EMBO J. 26(19):4215-27; Sandrin, V., et. al. (2013). Retrovirology. 10:104; Mercenne, G., et. al. (2015). Elife. 4; Zhai, Q., et. al. (2011). J Virol. 85(1):632-7).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to the first 716 amino acids of human ALIX.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Programmed cell death 6-interacting protein in human and murine cells.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • PDCD6IP
      • AIP1
      • ALIX
      • KIAA1375
      Purification MethodAffinity Purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Hela cell lysate.
      Western Blotting Analysis: A 1:500 dilution of this antibody detected ALIX in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC1435-25UL 04054839441561

      Documentation