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SCR139 Animal Free Collagenase/Dispase Blend I

Animal Free Collagenase/Dispase Blend I is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired.

Animal Free Collagenase/Dispase Blend I MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Harvesting, Cell Culture
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SCR139
50mg  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsSource
      Harv, CULTIsolated and purified from Clostridium histolyticum and Bacillus polymyxa cultures devoid of animal containing reagents.
      This product is manufactured in an ISO9001:2015 certified enviroment.
      Description
      Catalogue NumberSCR139
      DescriptionAnimal Free Collagenase/Dispase Blend I
      Alternate Names
      • MMP
      • Matrix metalloprotease
      • Dispase
      • Neutral Protease
      Background InformationCrude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types I, II, III, and IV or chromatographically purified collagenase. Dispase is especially suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. EMD Millipore’s Animal Free Collagenase/Dispase Blend I is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired. The product is prepared and purified from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens, such as BSE,TSE and other mammalian viruses, must be prevented.
      References
      Product Information
      PresentationProduct is diafiltered with water, filtered through a 0.22 micron membrane and lyophilized in vials.
      ActivityCollagenase Activity: ≥12,500 units per vial
      Caseinase Activity: ≥1000 caseinase units/mg
      Clostripain Activity: ≤5.0 clostripain units/mg
      Tryptic Activity: ≤0.5 trypic units/mg
      Quality LevelMQ200
      Applications
      ApplicationAnimal Free Collagenase/Dispase Blend I is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired.
      Key Applications
      • Cell Harvesting
      • Cell Culture
      Biological Information
      SourceIsolated and purified from Clostridium histolyticum and Bacillus polymyxa cultures devoid of animal containing reagents.
      This product is manufactured in an ISO9001:2015 certified enviroment.
      Physicochemical Information
      ActivityCollagenase Activity: ≥12,500 units per vial
      Caseinase Activity: ≥1000 caseinase units/mg
      Clostripain Activity: ≤5.0 clostripain units/mg
      Tryptic Activity: ≤0.5 trypic units/mg
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.
      Packaging Information
      Material Size50mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCR139 04053252982682

      Documentation

      Animal Free Collagenase/Dispase Blend I SDS

      Title

      Safety Data Sheet (SDS) 

      Animal Free Collagenase/Dispase Blend I Certificates of Analysis

      TitleLot Number
      Animal Free Collagenase/Dispase - VP1907051 VP1907051
      Animal Free Collagenase/Dispase Blend I - VP1903215 VP1903215
      Animal Free Collagenase/Dispase Blend I Purified Enzyme Blend VP1807102
      Animal Free Collagenase/Dispase Blend I Purified Enzyme Blend VP1704030

      Data Sheet

      Title
      Animal Free Collagenase/Dispase Blend I Purified Enzyme Blend