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SCM308 3dGRO™ Lung Organoid Maturation Medium

Serum-free ready-to-use media used to differentiate lung bud organoids (LBOs) into mature branching lung organoids.

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SCM308
250 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCM308
      Description3dGRO™ Lung Organoid Maturation Medium
      Alternate Names
      • Lung Bud Organoid Media
      • Lung Organoid Differentiation Media
      Background InformationThe lung epithelium undergoes a complex series of endoderm–mesoderm-mediated signaling events to generate a complex arborized network of conducting airway (bronchi, bronchioles) and gas-exchanging units (alveoli). Lung epithelium differentiation stages include: anterior/posterior and dorsal/ventral patterning, lung specification, lung budding, branching morphogenesis, and maturation.

      The 3dGRO™ Lung Organoid Maturation Medium is a chemically defined/serum-free ready-to-use media used to differentiate lung bud organoids (LBOs) into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures. Large numbers of mature lung organoids may be generated that express appropriate markers indicative of multiple cell types found in the mature lung and airways including: SFTPB and SFTPC, MUC5AC, EpCAM, Sox9, Nkx2.1, acetyl-a-tubulin and Vimentin. Additionally, the mature lung organoids express ACE2 and TMPRSS2 for SARS-CoV2 viral research. The 3dGRO™ Lung Organoid Maturation Medium is a component of the 3dGRO™ Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures. The 3dGRO™ Human Lung Organoid Culture System includes (all sold separately):

      • Definitive Endoderm Induction Medium (SCM302)
      • Anterior Foregut Endoderm Induction Medium I (SCM305)
      • Anterior Foregut Endoderm Induction Medium II (SCM306)
      • 3dGRO™ Lung Organoid Branching Medium (SCM307)
      • 3dGRO™ Lung Organoid Maturation Medium (SCM308)
      • Human iPSC Derived AFE Progenitors (SCC301)
      References
      Product Information
      Applications
      ApplicationSerum-free ready-to-use media used to differentiate lung bud organoids (LBOs) into mature branching lung organoids.
      Key Applications
      • Cell Culture
      • Stem Cell Culture
      Biological Information
      Media FormLiquid
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Appearance (Color): Clear/No Particlulates (Red Liquid)
      • Osmolality: Pass
      • pH: 7.0 – 7.4
      • Sterility Tested: No Growth/Pass
      • Endotoxin: <2 EU/mL
      • Mycoplasma: Negative
      • Functional Assay: Pass
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store at -20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly. Use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.
      Packaging Information
      Material Size250 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCM308 04061842079612