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345806 β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli

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345806
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345806-50MIU
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      Description
      OverviewRecombinant, Streptococcus pneumoniae β1,4-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,4-linked galactose. This enzyme is not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
      Note: 1 mU = 1 milliunit.
      Catalogue Number345806
      Brand Family Calbiochem®
      Synonymsβ1,4-D-Galactoside Galactohydrolase
      References
      ReferencesKierman, U.A., et al. 2004. Proteomincs 4, 1825.
      Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
      Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
      Product Information
      CAS number9031-11-2
      Activity≥1.5 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol <i>p</i>-nitrophenol from <i>p</i>-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.
      EC number3.2.1.23
      FormLiquid
      FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5. Sterile solution.
      Quality LevelMQ200
      Biological Information
      Specific Activity≥6 units/mg protein
      Physicochemical Information
      ContaminantsN-acetylglucosaminidase, α-galactosidase, α-mannosidase, neuraminidases, proteases: none detected. Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Catalogue Number GTIN
      345806-50MIU 04055977194081