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14-249M p38δ/SAPK4 Protein, active, 250 µg

Active, recombinant GST fusion protein fused to full length human p38δ/SAPK4, for use in Kinase Assays.

p38δ/SAPK4 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-249M
250 µg  Also available in 10 μg size (cat#14-249) and in bulk (cat# 14-249-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-249M
Brand Family Upstate
Trade Name
  • Upstate
Descriptionp38δ/SAPK4 Protein, active, 250 µg
OverviewRecombinant GST fusion protein fused to full length human p38δ/SAPK4

CHK3
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, recombinant GST fusion protein fused to full length human p38δ/SAPK4, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in E. coli
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.
Gene Symbol
  • MAPK13
  • MGC99536
  • PRKM13
  • SAPK4
  • p38delta
Protein Targetp38δ/SAPK4
Purification MethodAffinity chromatography on glutathione Sepharose™ beads after activation with SKK3/MEK6
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O15264 # Responds to activation by environmental stress and pro- inflammatory cytokines by phosphorylating downstream targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K.
COFACTOR: Magnesium.
SIZE: 365 amino acids; 42090 Da
TISSUE SPECIFICITY: Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.
DOMAIN: SwissProt: O15264 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight71kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of MBP in vitro
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -20°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-249) and in bulk (cat# 14-249-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-249M 04053252731075

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-249 p38δ/SAPK4 Protein, active, 10 µg

Product Families

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Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases