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109278 Wright's eosine methylene blue

for microscopy

Wright's eosine methylene blue MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
109278
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1092780025 Glass bottle 25 g
1092781000 Metal can 1 kg
Description
Catalogue Number109278
DescriptionWright's eosine methylene blue
OverviewWright’s eosin methylene blue - for microscopy staining dye, is a defined mixture of the dyes C.I. 52015 and C.I. 45380 for hematological staining procedures and is used in conjunction with eosin Y. The Wright’s eosin methylene blue solution can be used for analysis and diagnostics in the whole blood differentiation. The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor microscopy
Biological Information
Physicochemical Information
Bulk density520 kg/m3
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Statement(s)H317: May cause an allergic skin reaction.
H319: Causes serious eye irritation.
Precautionary Statement(s)P261: Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
P264: Wash skin thoroughly after handling.
P272: Contaminated work clothing should not be allowed out of the workplace.
P280: Wear protective gloves/ eye protection/ face protection.
P302 + P352: IF ON SKIN: Wash with plenty of water.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Signal WordWarning
Storage class10 - 13 Other liquids and solids
WGKWGK 3 highly hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Categories of dangerharmful, irritant
Product Usage Statements
Storage and Shipping Information
StorageStore at +5°C to +30°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Identity (UV/VIS-Spectrum)passes test
pH-value (0,01 %; methanol 50 %)6.0 - 7.0
Absorption maximum λ 1 (Methanol)648 - 651 nm
Absorption maximum λ 2 (Methanol)521 - 524 nm
Spec. Absorptivity A 1%/1cm
- λ1max; 0.0005 %; methanol; calculated on dried substance
1200 - 1400
- λ2max; 0.0005 %; methanol; calculated on dried substance650 - 850
TLC-Testpasses test
Loss on drying (105 °C)≤ 10 %
Suitability for microscopypasses test
Global Trade Item Number
Catalogue Number GTIN
1092780025 04022536113036
1092781000 04022536113043

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Alternative Products

Catalogue Number Description
101383 Wright's eosin methylene blue solution

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Catalogue Number Description
101383 Wright's eosin methylene blue solution

Accessories (Primary)

Catalogue Number Description
109468 Buffer tablets pH 7.2
111374 Buffer tablets pH 6.8

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Categories

IVD/OEM Materials & Reagents > Microscopy > Dyes for Microscopy > Other Dry Dyes & Dye Mixtures