Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

69026 T7•Tag® Antibody Agarose

T7•Tag® Antibody Agarose MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69026
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69026-3 Glass bottle 2 ml
Description
OverviewThe T7•Tag® Antibody Agarose is designed for rapid immunoaffinity purification of target proteins that carry the T7•Tag sequence (i.e., the amino terminal 11 aa of the T7 gene 10 protein). Purification is based on binding target proteins to T7•Tag monoclonal antibody that is covalently coupled to cross-linked agarose beads, washing away unbound proteins, and eluting at pH 2.2. Capacity will vary somewhat between different target proteins, but the beads are standardized to bind a minimum of 300 µg T7•Tag β-galactosidase per milliliter of settled resin. The beads can be used in either batch or column methods and can be recycled a minimum of five times without loss of binding activity.
Catalogue Number69026
Brand Family Novagen®
References
Product Information
Quality LevelMQ400
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69026-3 07790788057800

Documentation

T7•Tag® Antibody Agarose SDS

Title

Safety Data Sheet (SDS) 

T7•Tag® Antibody Agarose Certificates of Analysis

TitleLot Number
69026

Citations

Title
  • Hiroko Hayakawa, et al. (2007) Soluble ST2 blocks interleukin-33 signaling in allergic airway inflammation. Journal of Biological Chemistry 282, 26369-26380.
  • Neelima Sukumar, et al. (2007) Differential Bvg phase-dependent regulation and combinatorial role in pathogenesis of two Bordetella paralogs, BipA and BcfA. Journal of Bacteriology 189, 3695-3704.
  • Andrew J. Leger, et al. (2006) Blocking the protease-activated receptor 1-4 heterodimer in platelet-mediated thrombosis. Circulation 113, 1244-1254.
  • Peter T. Beernink, et al. (2005) Specificity of protein interactions mediated by BRCT domains of the XRCC1 DNA repair protein. Journal of Biological Chemistry 280, 30206-30213.
  • Steven A. Jacobs, et al. (2005) Soluble domains of telomerase reverse transcriptase identified by high-throughput screening. Protein Science 14, 2051-2058.
  • Arlene E. Kray, et al. (2005) Positive regulation of IB kinase signaling by protein serine/threonine phosphatase 2A. Journal of Biological Chemistry 280, 35974-35982.
  • Taruho S. Kuroda and Mitsunori Fukuda. (2005) Functional analysis of Slac2-c/MyRIP as a linker protein between melanosomes and myosin VIIa. Journal of Biological Chemistry 280, 28015-28022.
  • Takamasa Suzuki, et al. (2005) An Arabidopsis protein with a novel calcium-binding repeat sequence interacts with TONSOKU/MGOUN3/BRUSHY1 involved in meristem maintenance. Plant and Cell Physiology 46, 1452-1461.
  • Robert L. Del Vecchio and Nicholas K. Tonks. (2005) The conserved immunoglobulin domain controls the subcellular localization of the homophilic adhesion receptor protein-tyrosine phosphataseμ. Journal of Biological Chemistry 280, 1603-1612.
  • Wolf Wente, et al. (2005) Interactions with PDZ domain proteins PIST/GOPC and PDZK1 regulate intracellular sorting of the somatostatin receptor subtype 5. Journal of Biological Chemistry 280, 32419-32425.
  • Jennifer R. Ball, Christian Dimaano and Katharine S. Ullman. (2004) The RNA binding domain within the nucleoporin Nup153 associates preferentially with single-stranded RNA. RNA 10, 19-27.
  • Mitsunori Fukuda and Taruho S. Kuroda. (2004) Missense mutations in the globular tail of myosin-Va in dilute mice partially impair binding of Slac2-a/melanophilin. Journal of Cell Science 117, 583-591.
  • Michael H. Kagey, Tiffany A. Melhuish and David Wotton. (2003) The polycomb protein Pc2 is a SUMO E3. Cell 113, 127-137.
  • Taruho S. Kuroda, Hiroyoshi Ariga and Mitsunori Fukuda. (2003) The actin-binding domain of Slac2-a/melanophilin is required for melanosome distribution in melanocytes. Molecular and Cellular Biology 23, 5245-5255.
  • Emily Bernstein, et al. (2001) Role for bidentate ribonuclease in the initiation step of RNA interference. 409, 363-366.
    		•

    User Protocols

    Title
    TB125 T7•Tag® Affinity Purification Kit